Synapse - Differential sensitivity of glioma-versus lung cancer-specific EGFR mutations to EGFR kinase inhibitors

Differential sensitivity of glioma-versus lung cancer-specific EGFR mutations to EGFR kinase inhibitors Journal Article

Authors:	Vivanco, I.; Robins, H. I.; Rohle, D.; Campos, C.; Grommes, C.; Nghiemphu, P. L.; Kubek, S.; Oldrini, B.; Chheda, M. G.; Yannuzzi, N.; Tao, H.; Zhu, S. J.; Iwanami, A.; Kuga, D.; Dang, J. L.; Pedraza, A.; Brennan, C. W.; Heguy, A.; Liau, L. M.; Lieberman, F.; Yung, W. K. A.; Gilbert, M. R.; Reardon, D. A.; Drappatz, J.; Wen, P. Y.; Lamborn, K. R.; Chang, S. M.; Prados, M. D.; Fine, H. A.; Horvath, S.; Wu, N.; Lassman, A. B.; Deangelis, L. M.; Yong, W. H.; Kuhn, J. G.; Mischel, P. S.; Mehta, M. P.; Cloughesy, T. F.; Mellinghoff, I. K.
Article Title:	Differential sensitivity of glioma-versus lung cancer-specific EGFR mutations to EGFR kinase inhibitors
Abstract:	Activation of the epidermal growth factor receptor (EGFR) in glioblastoma (GBM) occurs through mutations or deletions in the extracellular (EC) domain. Unlike lung cancers with EGFR kinase domain (KD) mutations, GBMs respond poorly to the EGFR inhibitor erlotinib. Using RNAi, we show that GBM cells carrying EGFR EC mutations display EGFR addiction. In contrast to KD mutants found in lung cancer, glioma-specific EGFR EC mutants are poorly inhibited by EGFR inhibitors that target the active kinase conformation (e.g., erlotinib). Inhibitors that bind to the inactive EGFR conformation, however, potently inhibit EGFR EC mutants and induce cell death in EGFR-mutant GBM cells. Our results provide first evidence for single kinase addiction in GBM and suggest that the disappointing clinical activity of first-generation EGFR inhibitors in GBM versus lung cancer may be attributed to the different conformational requirements of mutant EGFR in these 2 cancer types. SIGNIFICANCE: Approximately 40% of human glioblastomas harbor oncogenic EGFR alterations, but attempts to therapeutically target EGFR with first-generation EGFR kinase inhibitors have failed. Here, we demonstrate selective sensitivity of glioma-specific EGFR mutants to ATP-site competitive EGFR kinase inhibitors that target the inactive conformation of the catalytic domain. Cancer Discov; 2(5); 458-71. (c) 2012 AACR.
Keywords:	glioblastoma; gefitinib; tyrosine kinase; therapy; lapatinib; malignant gliomas; 3t3 cells; epidermal-growth-factor; tumor-cells; factor receptor inhibitors
Journal Title:	Cancer Discovery
Volume:	2
Issue:	5
ISSN:	2159-8274
Publisher:	American Association for Cancer Research
Date Published:	2012-05-01
Start Page:	458
End Page:	471
Language:	English
ACCESSION:	WOS:000306328600039
DOI:	10.1158/2159-8290.cd-11-0284
PROVIDER:	wos
PMCID:	PMC3354723
PUBMED:	22588883
Notes:	--- - Article - "Source: Wos"