Molecular profiling of oral microbiota in jawbone samples of bisphosphonate-related osteonecrosis of the jaw Journal Article


Authors: Wei, X.; Pushalkar, S.; Estilo, C.; Wong, C.; Farooki, A.; Fornier, M.; Bohle, G.; Huryn, J.; Li, Y.; Doty, S.; Saxena, D.
Article Title: Molecular profiling of oral microbiota in jawbone samples of bisphosphonate-related osteonecrosis of the jaw
Abstract: Objective: Infection has been hypothesized as a contributing factor to bisphosphonate (BP)-related osteonecrosis of the jaw (BRONJ). The objective of this study was to determine the bacterial colonization of jawbone and identify the bacterial phylotypes associated with BRONJ. Materials and methods: Culture-independent 16S rRNA gene-based molecular techniques were used to determine and compare the total bacterial diversity in bone samples collected from 12 patients with cancer (six, BRONJ with history of BP; six, controls without BRONJ, no history of BP but have infection). Results: Denaturing gradient gel electrophoresis profile and Dice coefficient displayed a statistically significant clustering of profiles, indicating different bacterial population in BRONJ subjects and control. The top three genera ranked among the BRONJ group were Streptococcus (29%), Eubacterium (9%), and Pseudoramibacter (8%), while in the control group were Parvimonas (17%), Streptococcus (15%), and Fusobacterium (15%). H&E sections of BRONJ bone revealed layers of bacteria along the surfaces and often are packed into the scalloped edges of the bone. Conclusion: This study using limited sample size indicated that the jawbone associated with BRONJ was heavily colonized by specific oral bacteria and there were apparent differences between the microbiota of BRONJ and controls. © 2012 John Wiley & Sons A/S.
Keywords: adult; human tissue; aged; nonhuman; nucleotide sequence; bacterium detection; bacterial colonization; unindexed sequence; dna extraction; genomic dna; bacterium; phylogeny; osteonecrosis of the jaw; jaw osteonecrosis; osteomyelitis; bisphosphonate; jaw; eubacterium; fusobacterium; mouth flora; parvimonas; 16s rdna; denaturing gradient gel electrophoresis; microbial shift; lactobacillus gasseri; pseudoramibacter alactolyticus; streptococcus mitis
Journal Title: Oral Diseases
Volume: 18
Issue: 6
ISSN: 1354-523X
Publisher: Wiley Blackwell  
Date Published: 2012-09-01
Start Page: 602
End Page: 612
Language: English
DOI: 10.1111/j.1601-0825.2012.01916.x
PROVIDER: scopus
PUBMED: 22443347
PMCID: PMC7167636
DOI/URL:
Notes: --- - "Export Date: 4 September 2012" - "CODEN: ORDIF" - "Source: Scopus"
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MSK Authors
  1. Azeez Farooki
    63 Farooki
  2. Joseph M Huryn
    94 Huryn
  3. George C Bohle
    17 Bohle
  4. Cherry Estilo
    77 Estilo
  5. Monica Nancy Fornier
    153 Fornier