Correlation of a priori DCE-MRI and 1H-MRS data with molecular markers in neck nodal metastases: Initial analysis Journal Article

Authors: Jansen, J. F. A.; Carlson, D. L.; Lu, Y.; Stambuk, H. E.; Moreira, A. L.; Singh, B.; Patel, S. G.; Kraus, D. H.; Wong, R. J.; Shaha, A. R.; Shah, J. P.; Shukla-Dave, A.
Article Title: Correlation of a priori DCE-MRI and 1H-MRS data with molecular markers in neck nodal metastases: Initial analysis
Abstract: The aim of the present study is to correlate non-invasive, pretreatment biological imaging (dynamic contrast enhanced-MRI [DCE-MRI] and proton magnetic resonance spectroscopy [ 1H-MRS]) findings with specific molecular marker data in neck nodal metastases of head and neck squamous cell carcinoma (HNSCC) patients. Pretreatment DCE-MRI and 1H-MRS were performed on neck nodal metastases of 12 patients who underwent surgery. Surgical specimens were analyzed with immunohistochemistry (IHC) assays for: Ki-67 (reflecting cellular proliferation), vascular endothelial growth factor (VEGF) (the "endogenous marker" of tumor vessel growth), carbonic anhydrase (CAIX), hypoxia inducible transcription factor (HIF-1α), and human papillomavirus (HPV). Additionally, necrosis was estimated based on H&E staining. The Spearman correlation was used to compare DCE-MRI, 1H-MRS, and molecular marker data. A significant correlation was observed between DCE-MRI parameter std(k ep) and VEGF IHC expression level (rho = 0.81, p = 0.0001). Furthermore, IHC expression levels of Ki-67 inversely correlated with std(K trans) and std(v e) (rho = -0.71; p = 0.004, and rho = -0.73; p = 0.003, respectively). Other DCE-MRI, 1H-MRS and IHC values did not show significant correlation. The results of this preliminary study indicate that the level of heterogeneity of perfusion in metastatic HNSCC seems positively correlated with angiogenesis, and inversely correlated with proliferation. These results are preliminary in nature and are indicative, and not definitive, trends portrayed in HNSCC patients with nodal disease. Future studies with larger patient populations need to be carried out to validate and clarify our preliminary findings. © 2012 Elsevier Ltd. All rights reserved.
Keywords: immunohistochemistry; vasculotropin; adult; clinical article; human tissue; protein expression; aged; middle aged; vascular endothelial growth factor a; cancer surgery; carcinoma, squamous cell; conference paper; nuclear magnetic resonance imaging; magnetic resonance imaging; lymph node metastasis; cancer diagnosis; lymph nodes; lymphatic metastasis; ki 67 antigen; cell proliferation; ki-67 antigen; tumor markers, biological; molecular marker; magnetic resonance spectroscopy; contrast media; neck; hypoxia inducible factor 1alpha; mouth neoplasms; dce-mri; proton nuclear magnetic resonance; hypoxia-inducible factor 1, alpha subunit; wart virus; molecular markers; carbonate dehydratase; carbonic anhydrases; head and neck squamous cell carcinoma; human papillomavirus 16; dynamic contrast enhanced nuclear magnetic resonance imaging; 1h-mrs
Journal Title: Oral Oncology
Volume: 48
Issue: 8
ISSN: 1368-8375
Publisher: Elsevier Inc.  
Date Published: 2012-08-01
Start Page: 717
End Page: 722
Language: English
DOI: 10.1016/j.oraloncology.2012.02.001
PROVIDER: scopus
PMCID: PMC3368067
PUBMED: 22366441
Notes: --- - "Export Date: 4 September 2012" - "CODEN: EJCCE" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Dennis Kraus
    268 Kraus
  2. Ashok R Shaha
    643 Shaha
  3. Bhuvanesh Singh
    233 Singh
  4. Snehal G Patel
    378 Patel
  5. Diane Carlson
    52 Carlson
  6. Andre L Moreira
    175 Moreira
  7. Hilda Stambuk
    47 Stambuk
  8. Jacobus Jansen
    14 Jansen
  9. Richard J Wong
    341 Wong
  10. Yonggang Lu
    11 Lu
  11. Amita Dave
    117 Dave
  12. Jatin P Shah
    685 Shah