Further phenotypic characterization of the primitive lineage- CD34+CD38- CD90+CD45RA- Hematopoietic stem cell/progenitor cell sub-population isolated from cord blood, mobilized peripheral blood and patients with chronic myelogenous leukemia Journal Article


Authors: Wisniewski, D.; Affer, M.; Wilshire, J.; Clarkson, B.
Article Title: Further phenotypic characterization of the primitive lineage- CD34+CD38- CD90+CD45RA- Hematopoietic stem cell/progenitor cell sub-population isolated from cord blood, mobilized peripheral blood and patients with chronic myelogenous leukemia
Abstract: The most primitive hematopoietic stem cell (HSC)/progenitor cell (PC) population reported to date is characterized as being Lin-CD34+CD38-CD90+CD45R. We have a long-standing interest in comparing the characteristics of hematopoietic progenitor cell populations enriched from normal subjects and patients with chronic myelogenous leukemia (CML). In order to investigate further purification of HSCs and for potential targetable differences between the very primitive normal and CML stem/PCs, we have phenotypically compared the normal and CML Lin-CD34+CD38-CD90+CD45RA- HSC/ PC populations. The additional antigens analyzed were HLADR, the receptor tyrosine kinases c-kit and Tie2, the interleukin- 3 cytokine receptor, CD33 and the activation antigen CD69, the latter of which was recently reported to be selectively elevated in cell lines expressing the Bcr-Abl tyrosine kinase. Notably, we found a strikingly low percentage of cells from the HSC/PC sub-population isolated from CML patients that were found to express the c-kit receptor (<1%) compared with the percentages of HSC/PCs expressing the c-kitR isolated from umbilical cord blood (50%) and mobilized peripheral blood (10%). Surprisingly, Tie2 receptor expression within the HSC/PC subset was extremely low from both normal and CML samples. Using in vivo transplantation studies, we provide evidence that HLA-DR, c-kitR, Tie2 and IL-3R may not be suitable markers for further partitioning of HSCs from the Lin- CD34+CD38-CD90+CD45RA- sub-population. © 2011 Macmillan Publishers Limited All rights reserved.
Keywords: phenotype; stem cells; normal; myeloid; leukemic
Journal Title: Blood Cancer Journal
Volume: 1
Issue: 9
ISSN: 2044-5385
Publisher: Nature Publishing Group  
Date Published: 2011-09-01
Start Page: e36
Language: English
DOI: 10.1038/bcj.2011.35
PROVIDER: scopus
PMCID: PMC3255253
PUBMED: 22829197
DOI/URL:
Notes: --- - "Export Date: 1 August 2012" - "Source: Scopus"
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MSK Authors
  1. Maurizio Affer
    1 Affer