Assay for isolation of inhibitors of her2-kinase expression Journal Article
| Authors: | Chiosis, G.; Keeton, A. B. |
| Article Title: | Assay for isolation of inhibitors of her2-kinase expression |
| Abstract: | Her2 (ErbB2) protein is overexpressed in breast and other solid tumors, and its expression is associated with progressive disease. Current therapies directed toward Her2 either block dimerization of the receptor or inhibit tyrosine kinase activity to disrupt intracellular signaling. However, little is known about alternative mechanisms for suppressing Her2 expression, possibly by inducing degradation or blocking synthesis. Here, we describe a hybrid western-blotting and enzyme-linked immunosorbent assay (ELISA) designed to identify in low- to medium-throughput format noncytotoxic compounds that reduce expression of Her2 protein. |
| Keywords: | genetics; antineoplastic agents; methodology; antineoplastic agent; metabolism; epidermal growth factor receptor 2; drug screening; enzymology; pathology; tumor cell culture; tumor cells, cultured; drug evaluation, preclinical; breast neoplasms; enzyme linked immunosorbent assay; blotting, western; drug antagonism; breast tumor; bioassay; western blotting; receptor, erbb-2; biological assay; enzyme-linked immunosorbent assay |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 486 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2009-01-01 |
| Start Page: | 139 |
| End Page: | 149 |
| Language: | English |
| DOI: | 10.1007/978-1-60327-545-3_10 |
| PUBMED: | 19347621 |
| PROVIDER: | scopus |
| PMCID: | PMC2732334 |
| DOI/URL: | |
| Notes: | Chapter 10 in "Cell-Based Assays for High-Throughput Screening: Methods and Protocols" (ISBN: 978-1-60327-544-6)--- Export Date: 30 November 2010 - Source: Scopus |
