Will a peripheral blood (PB) sample yield the same diagnostic and prognostic cytogenetic data as the concomitant bone marrow (BM) in myelodysplasia? Journal Article
| Authors: | Cherry, A. M.; Slovak, M. L.; Campbell, L. J.; Chun, K.; Eclache, V.; Haase, D.; Haferlach, C.; Hildebrandt, B.; Iqbal, A. M.; Jhanwar, S. C.; Ohyashiki, K.; Sole, F.; Vandenberghe, P.; VanDyke, D. L.; Zhang, Y.; Dewald, G. W. |
| Article Title: | Will a peripheral blood (PB) sample yield the same diagnostic and prognostic cytogenetic data as the concomitant bone marrow (BM) in myelodysplasia? |
| Abstract: | In patients with myelodysplastic syndromes (MDS), chromosome anomalies are detected by conventional cytogenetic studies (CCS) and/or interphase fluorescence in situ hybridization (FISH) of bone marrow (BM) samples and provide prognostic and diagnostic information, which can direct therapy. Whether peripheral blood (PB) can be substituted for bone marrow in these cases and can provide the same information remains unknown. Concurrent BM and PB specimens collected from 100 patients with recently diagnosed MDS were studied using both CCS and FISH. While 68% of BM samples showed an abnormal karyotype by CCS, only 31% of PB samples were abnormal by CCS. In 12% of patients, FISH and CCS were discordant due to the inability of the FISH panel to detect all possible abnormalities. However, only one case (1%) had a cryptic abnormality detected by FISH. BM and PB FISH were discordant in 3% of cases, most likely due to the smaller clone size in PB vs. BM. While PB should not be substituted for BM at diagnosis, it is a viable alternative for monitoring patients using the appropriate FISH probe(s). © 2012 Elsevier Ltd. |
| Keywords: | adolescent; adult; child; school child; aged; aged, 80 and over; middle aged; young adult; major clinical study; disease course; prospective study; models, biological; bone marrow; cytogenetics; patient monitoring; chromosome aberration; myelodysplastic syndrome; fluorescence in situ hybridization; blood sampling; biopsy, needle; medical oncology; fish; chromosome analysis; karyotyping; molecular probe; cytogenetic analysis; myelodysplastic syndromes; bone marrow examination; mds; refractory anemia; hematologic tests; analytical parameters; peripheral blood; refractory anemia with excess blasts; refractory anemia with ringed sideroblasts; 5q- syndrome; clone size; refractory cytopenia with multilineage dysplasia |
| Journal Title: | Leukemia Research |
| Volume: | 36 |
| Issue: | 7 |
| ISSN: | 0145-2126 |
| Publisher: | Elsevier Ltd |
| Date Published: | 2012-07-01 |
| Start Page: | 832 |
| End Page: | 840 |
| Language: | English |
| DOI: | 10.1016/j.leukres.2012.03.013 |
| PROVIDER: | scopus |
| PUBMED: | 22537394 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 2 July 2012" - "CODEN: LERED" - "Source: Scopus" |
