siRNA off-target effects in genome-wide screens identify signaling pathway members Journal Article


Authors: Buehler, E.; Khan, A. A.; Marine, S.; Rajaram, M.; Bahl, A.; Burchard, J.; Ferrer, M.
Article Title: siRNA off-target effects in genome-wide screens identify signaling pathway members
Abstract: We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This technique can be applied to siRNA screen results retroactively to confirm positives and identify genes missed using conventional methods for on-target gene selection.
Journal Title: Scientific Reports
Volume: 2
ISSN: 2045-2322
Publisher: Nature Publishing Group  
Date Published: 2012-01-01
Language: English
DOI: 10.1038/srep00428
PROVIDER: scopus
PMCID: PMC3361704
PUBMED: 22645644
DOI/URL:
Notes: --- - "Export Date: 2 July 2012" - "Source: Scopus"
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  1. Aly Azeem Khan
    8 Khan