Involved in De Novo 2-containing complex involved in RNA-directed DNA methylation in Arabidopsis Journal Article
| Authors: | Ausin, I.; Greenberg, M. V. C.; Simanshu, D. K.; Hale, C. J.; Vashisht, A. A.; Simon, S. A.; Lee, T. F.; Feng, S.; Española, S. D.; Meyers, B. C.; Wohlschlegel, J. A.; Patel, D. J.; Jacobsen, S. E. |
| Article Title: | Involved in De Novo 2-containing complex involved in RNA-directed DNA methylation in Arabidopsis |
| Abstract: | At least three pathways control maintenance of DNA cytosine methylation in Arabidopsis thaliana. However, the RNA-directed DNA methylation (RdDM) pathway is solely responsible for establishment of this silencing mark. We previously described INVOLVED IN DE NOVO 2 (IDN2) as being an RNA-binding RdDM component that is required for DNA methylation establishment. In this study, we describe the discovery of two partially redundant proteins that are paralogous to IDN2 and that form a stable complex with IDN2 in vivo. Null mutations in both genes, termed IDN2-LIKE 1 and IDN2-LIKE 2 (IDNL1 and IDNL2), result in a phenotype that mirrors, but does not further enhance, the idn2 mutant phenotype. Genetic analysis suggests that this complex acts in a step in the downstream portion of the RdDM pathway. We also have performed structural analysis showing that the IDN2 XS domain adopts an RNA recognition motif (RRM) fold. Finally, genome-wide DNA methylation and expression analysis confirms the placement of the IDN proteins in an RdDM pathway that affects DNA methylation and transcriptional control at many sites in the genome. Results from this study identify and describe two unique components of the RdDM machinery, adding to our understanding of DNA methylation control in the Arabidopsis genome. |
| Keywords: | controlled study; protein expression; unclassified drug; gene mutation; mutation; nonhuman; genetic analysis; protein conformation; protein domain; mass spectrometry; phenotype; protein binding; rna interference; in vivo study; dna methylation; rna; rna-binding proteins; blotting, western; dna; transcription regulation; amino acid sequence; molecular sequence data; sequence homology, amino acid; epigenetics; genomics; binding protein; models, molecular; protein structure, tertiary; multiprotein complexes; binding sites; protein structure; blotting, northern; transcriptome; protein structure, secondary; arabidopsis; sirnas; arabidopsis thaliana; arabidopsis proteins; plant leaves; gene expression regulation, plant; plants, genetically modified; rna, plant; involved in de novo 2 like 1 protein; involved in de novo 2 like 2 protein; involved in de novo 2 protein; plant genome; rna directed dna methylation; flowers |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 109 |
| Issue: | 22 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2012-05-29 |
| Start Page: | 8374 |
| End Page: | 8381 |
| Language: | English |
| DOI: | 10.1073/pnas.1206638109 |
| PROVIDER: | scopus |
| PMCID: | PMC3365198 |
| PUBMED: | 22592791 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 July 2012" - "CODEN: PNASA" - "Source: Scopus" |
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