Enzymology of RNA cap synthesis Journal Article
| Authors: | Ghosh, A.; Lima, C. D. |
| Article Title: | Enzymology of RNA cap synthesis |
| Abstract: | The 5' guanine-N7 methyl cap is unique to cellular and viral messenger RNA (mRNA) and is the first co-transcriptional modification of mRNA. The mRNA cap plays a pivotal role in mRNA biogenesis and stability, and is essential for efficient splicing, mRNA export, and translation. Capping occurs by a series of three enzymatic reactions that results in formation of N7-methyl guanosine linked through a 5'-5' inverted triphosphate bridge to the first nucleotide of a nascent transcript. Capping of cellular mRNA occurs co-transcriptionally and in vivo requires that the capping apparatus be physically associated with the RNA polymerase II elongation complex. Certain capped mRNAs undergo further methylation to generate distinct cap structures. Although mRNA capping is conserved among viruses and eukaryotes, some viruses have adopted strategies for capping mRNA that are distinct from the cellular mRNA capping pathway. 2010 John Wiley & Sons, Ltd. |
| Keywords: | genetics; review; animal; metabolism; animals; biological model; models, biological; acid anhydride hydrolase; rna triphosphatase; acid anhydride hydrolases; physiology; biosynthesis; chemistry; messenger rna; virus rna; rna caps; rna, messenger; models, molecular; chemical structure; conformation; nucleic acid conformation; rna processing; enzymes; rna, viral; rna processing, post-transcriptional; enzyme; nucleotidyltransferase; capped rna; nucleotidyltransferases; messenger ribonucleic acid guanylyltransferase |
| Journal Title: | Wiley Interdisciplinary Reviews: RNA |
| Volume: | 1 |
| Issue: | 1 |
| ISSN: | 1757-7012 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 2010-07-01 |
| Start Page: | 152 |
| End Page: | 172 |
| Language: | English |
| PUBMED: | 21956912 |
| PROVIDER: | scopus |
| DOI: | 10.1002/wrna.19 |
| PMCID: | PMC3962952 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 5 June 2012" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work