A role for neuronal piRNAs in the epigenetic control of memory-related synaptic plasticity Journal Article

   

Authors:	Rajasethupathy, P.; Antonov, I.; Sheridan, R.; Frey, S.; Sander, C.; Tuschl, T.; Kandel, E. R.
Article Title:	A role for neuronal piRNAs in the epigenetic control of memory-related synaptic plasticity
Abstract:	Small RNA-mediated gene regulation during development causes long-lasting changes in cellular phenotypes. To determine whether small RNAs of the adult brain can regulate memory storage, a process that requires stable and long-lasting changes in the functional state of neurons, we generated small RNA libraries from the Aplysia CNS. In these libraries, we discovered an unexpectedly abundant expression of a 28 nucleotide sized class of piRNAs in brain, which had been thought to be germline specific. These piRNAs have unique biogenesis patterns, predominant nuclear localization, and robust sensitivity to serotonin, a modulatory transmitter that is important for memory. We find that the Piwi/piRNA complex facilitates serotonin-dependent methylation of a conserved CpG island in the promoter of CREB2, the major inhibitory constraint of memory in Aplysia, leading to enhanced long-term synaptic facilitation. These findings provide a small RNA-mediated gene regulatory mechanism for establishing stable long-term changes in neurons for the persistence of memory. © 2012 Elsevier Inc.
Keywords:	controlled study; methylation; promoter region; nonhuman; sensitivity analysis; animal cell; phenotype; animals; animal tissue; nerve tissue proteins; rna, small interfering; neurons; gene expression regulation; molecular sequence data; epigenetics; cpg island; nerve cell plasticity; base sequence; gene control; gene regulatory network; memory; nuclear localization signal; activating transcription factor 4; piwi interacting rna; biogenesis; serotonin; aplysia; neuronal plasticity; synapses; epigenomics; germline micronucleus
Journal Title:	Cell
Volume:	149
Issue:	3
ISSN:	0092-8674
Publisher:	Cell Press
Date Published:	2012-04-27
Start Page:	693
End Page:	707
Language:	English
DOI:	10.1016/j.cell.2012.02.057
PROVIDER:	scopus
PUBMED:	22541438
PMCID:	PMC3442366
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84860320656&partnerID=40&md5=53c5637e028f47a83f49789ad8b2ced9
Notes:	--- - "Cited By (since 1996): 1" - "Export Date: 4 June 2012" - "CODEN: CELLB" - "Source: Scopus"

https://synapse.mskcc.org/synapse/works/has_related_data_chk/28528