Formation of dAMP-glycerol and dAMP-Tris derivatives by Thermococcus kodakaraensis DNA primase Journal Article
| Authors: | Galal, W. C.; Pan, M.; Giulian, G.; Yuan, W.; Li, S.; Edwards, J. L.; Marino, J. P.; Kelman, Z.; Hurwitz, J. |
| Article Title: | Formation of dAMP-glycerol and dAMP-Tris derivatives by Thermococcus kodakaraensis DNA primase |
| Abstract: | In the presence of dATP, glycerol, and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis catalyzed the formation of dAMP and two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the T. kodakaraensis p41 catalytic subunit alone and the T. kodakaraensis p41-p46 complex in the absence of a DNA template. They were not formed with preparations containing the catalytically inactive p41 subunit. Similar glycerol and Tris derivatives as well as dNMPs were also formed with dGTP, dCTP, or dTTP. The mechanism contributing to the formation of these products and its implications in the initiation reaction catalyzed by the T. kodakaraensis primase are discussed. |
| Keywords: | controlled study; unclassified drug; nonhuman; mass spectrometry; complex formation; dna; high performance liquid chromatography; catalysis; proton nuclear magnetic resonance; archaeal proteins; dna-template; glycerol; thermococcus kodakarensis; thermococcus; catalytic subunits; dna primase; deoxycytidine triphosphate; protein p46; deoxyguanosine triphosphate; initiation reactions; thermococcus kodakaraensis; tris buffers; two-product; deoxyadenosine phosphate; protein p41; thymidine triphosphate; trometamol; deoxyadenine nucleotides; tromethamine |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 287 |
| Issue: | 20 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2012-05-11 |
| Start Page: | 16220 |
| End Page: | 16229 |
| Language: | English |
| DOI: | 10.1074/jbc.M111.338160 |
| PROVIDER: | scopus |
| PMCID: | PMC3351308 |
| PUBMED: | 22427647 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 4 June 2012" - "CODEN: JBCHA" - "Source: Scopus" |
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