Ex-vivo analysis of CD8+ T cells infiltrating colorectal tumors identifies a major effector-memory subset with low perforin content Journal Article
| Authors: | Ye, S. W.; Wang, Y.; Valmori, D.; Ayyoub, M.; Han, Y.; Xu, X. L.; Zhao, A. L.; Qu, L.; Gnjatic, S.; Ritter, G.; Old, L. J.; Gu, J. |
| Article Title: | Ex-vivo analysis of CD8+ T cells infiltrating colorectal tumors identifies a major effector-memory subset with low perforin content |
| Abstract: | Previous studies have indicated that the infiltration of CD8+ T cells in colorectal cancer is an independent predictor of increased survival but clinical observations have suggested that the cytotoxic function of CD8+ T cells infiltrating colorectal cancer may often be limited. In this study, we have assessed the phenotype of colorectal cancer CD8+ tumor-infiltrating lymphocytes (TILs) isolated ex vivo from tumor tissue, and assessed the perforin content of TIL with respect to their location using immunohistochemistry. We found that CD8+ T cells TILs isolated from colorectal cancer are mainly composed of antigen-experienced cells of effector memory type (TEM, CD45RA-CCR7-, and CD27+/CD28- or CD27-/CD28-), and contain only minor proportions of terminally differentiated CD8+ T cells (TEMRA, CD45RA+CCR7-). The perforin content of these TILs, however, is significantly lower than that of antigen-experienced T cells in PBMCs due to the much lower levels of perforin found in the CD27-CD28- subset in TILs compared with CD8+ T cells of similar phenotype in PBMCs. © 2006 Springer Science+Business Media, Inc. |
| Keywords: | immunohistochemistry; adult; clinical article; aged; aged, 80 and over; middle aged; flow cytometry; colorectal cancer; adenocarcinoma; cd8 antigen; cd8+ t lymphocyte; tumor associated leukocyte; cd8-positive t-lymphocytes; lymphocytes, tumor-infiltrating; phenotype; cell infiltration; cell differentiation; colorectal neoplasms; statistical significance; membrane glycoproteins; colorectal tumor; perforin; effector cell; t-lymphocyte subsets; memory cell; chemokine receptor ccr7; ex vivo study; cd28 antigen; immunologic memory; peripheral blood mononuclear cell; cd27 antigen; cd45ra antigen; tumor infiltrating lymphocytes; pore forming cytotoxic proteins; protein content |
| Journal Title: | Journal of Clinical Immunology |
| Volume: | 26 |
| Issue: | 5 |
| ISSN: | 0271-9142 |
| Publisher: | Springer |
| Date Published: | 2006-09-01 |
| Start Page: | 447 |
| End Page: | 456 |
| Language: | English |
| DOI: | 10.1007/s10875-006-9040-4 |
| PUBMED: | 16967323 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 6" - "Export Date: 4 June 2012" - "CODEN: JCIMD" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work