A peptidomimetic siRNA transfection reagent for highly effective gene silencing Journal Article
| Authors: | Utku, Y.; Dehan, E.; Ouerfelli, O.; Piano, F.; Zuckermann, R. N.; Pagano, M.; Kirshenbaum, K. |
| Article Title: | A peptidomimetic siRNA transfection reagent for highly effective gene silencing |
| Abstract: | RNA interference (RNAi) techniques hold forth great promise for therapeutic silencing of deleterious genes. However, clinical applications of RNAi require the development of safe and efficient methods for intracellular delivery of small interfering RNA (siRNA) oligonucleotides specific to targeted genes. We describe the use of a lipitoid, a cationic oligopeptoid-phospholipid conjugate, for non-viral transfection of synthetic siRNA oligos in cell culture. This peptidomimetic delivery vehicle allows for efficient siRNA transfection in a variety of human cell lines with negligible toxicity and promotes extensive downregulation of the targeted genes at both the protein and the mRNA level. We compare the lipitoid reagent to a standard commercial transfection reagent. The lipitoid is highly efficient even in primary IMR-90 human lung fibroblasts in which other commercial reagents are typically ineffective. © The Royal Society of Chemistry 2006. |
| Keywords: | genetics; comparative study; methodology; metabolism; cells, cultured; lipid; small interfering rna; rna, small interfering; rna interference; hela cell; cell line, tumor; hela cells; transfection; physiology; drug delivery systems; gene expression regulation; chemistry; genetic transfection; cell culture; tumor cell line; fibroblast; fibroblasts; lipids; gene silencing; phosphatidylethanolamine; phosphatidylethanolamines; oligopeptides; liposome; drug delivery system; liposomes; cation; oligopeptide; cations; lipofectamine |
| Journal Title: | Molecular BioSystems |
| Volume: | 2 |
| Issue: | 6 |
| ISSN: | 1742-206X |
| Publisher: | Royal Society of Chemistry |
| Date Published: | 2006-06-01 |
| Start Page: | 312 |
| End Page: | 317 |
| Language: | English |
| DOI: | 10.1039/b603229j |
| PUBMED: | 16880950 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 19" - "Export Date: 4 June 2012" - "Source: Scopus" |
