Squamous cell carcinoma related oncogene/DCUN1D1 is highly conserved and activated by amplification in squamous cell carcinomas Journal Article


Authors: Sarkaria, I.; O-Charoenrat, P.; Talbot, S. G.; Reddy, P. G.; Ngai, I.; Maghami, E.; Patel, K. N.; Lee, B.; Yonekawa, Y.; Dudas, M.; Kaufman, A.; Ryan, R.; Ghossein, R.; Rao, P. H.; Stoffel, A.; Ramanathan, Y.; Singh, B.
Article Title: Squamous cell carcinoma related oncogene/DCUN1D1 is highly conserved and activated by amplification in squamous cell carcinomas
Abstract: Chromosomal amplification at 3q is common to multiple human cancers, but has a specific predilection for squamous cell carcinomas (SCC) of mucosal origin. We identified and characterized a novel oncogene, SCC-related oncogene (SCCRO), which is amplified along the 3q26.3 region in human SCC. Amplification and overexpression of SCCRO in these tumors correlate with poor clinical outcome. The importance of SCCRO amplification in malignant transformation is established by the apoptotic response to short hairpin RNA against SCCRO, exclusively in cancer cell lines carrying SCCRO amplification. The oncogenic potential of SCCRO is underscored by its ability to transform fibroblasts (NIH-3T3 cells) in vitro and in vivo. We show that SCCRO regulates Gli1-a key regulator of the hedgehog (HH) pathway. Collectively, these data suggest that SCCRO is a novel component of the HH signaling pathway involved in the malignant transformation of squamous cell lineage. ©2006 American Association for Cancer Research.
Keywords: signal transduction; controlled study; human tissue; promoter region; squamous cell carcinoma; carcinoma, squamous cell; nonhuman; genetic analysis; animal cell; mouse; animals; mice; cell survival; gene targeting; gene overexpression; apoptosis; gene amplification; neoplasm proteins; sonic hedgehog protein; animal experiment; animal model; hedgehog proteins; rna, small interfering; in vivo study; cancer cell culture; in vitro study; cell line, tumor; mice, inbred balb c; transcription factors; cell transformation, neoplastic; oncogenes; molecular cloning; cloning, molecular; oncogene; gene activation; gene expression regulation, neoplastic; recombinant fusion proteins; mice, nude; cell transformation; oncogene proteins; fibroblast; gene control; neoplasm transplantation; transcription factor gli1; short hairpin rna; genetic conservation; nih 3t3 cells; chromosome 3q; chromosomes, human, pair 3; squamous cell carcinoma related oncogene
Journal Title: Cancer Research
Volume: 66
Issue: 19
ISSN: 0008-5472
Publisher: American Association for Cancer Research  
Date Published: 2006-10-01
Start Page: 9437
End Page: 9444
Language: English
DOI: 10.1158/0008-5472.can-06-2074
PUBMED: 17018598
PROVIDER: scopus
DOI/URL:
Notes: --- - "Cited By (since 1996): 17" - "Export Date: 4 June 2012" - "CODEN: CNREA" - "Source: Scopus"
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Citation Impact
MSK Authors
  1. Pabbathi G Reddy
    8 Reddy
  2. Simon G Talbot
    26 Talbot
  3. Kepal N Patel
    13 Patel
  4. Ellie Ghaem Maghami
    11 Maghami
  5. Russell J H Ryan
    5 Ryan
  6. Ronald A Ghossein
    353 Ghossein
  7. Bhuvanesh Singh
    222 Singh
  8. Maria E Dudas
    53 Dudas
  9. Ivan Ngai
    17 Ngai
  10. Benjamin Lee
    3 Lee