Profiling bladder cancer using targeted antibody arrays Journal Article
| Authors: | Sanchez Carbayo, M.; Socci, N. D.; Lozano, J. J.; Haab, B. B.; Cordon-Cardo, C. |
| Article Title: | Profiling bladder cancer using targeted antibody arrays |
| Abstract: | Bladder cancer is a common malignancy requiring a high degree of surveillance because of the frequent recurrences and the poor clinical outcome of invasive disease. To date, serum biomarkers for bladder cancer lack optimal sensitivity and specificity to assist in diagnosis and disease categorization. Here, we designed antibody arrays for bladder cancer by selecting antibodies against targets differentially expressed to bladder tumors. Serum protein profiles measured by an antibody array containing 254 antibodies discriminated bladder cancer patients from controls (n = 95) with a correct classification rate of 93-7%. A second independent antibody array containing 144 antibodies revealed that protein profiles provide predictive information by stratifying patients with bladder tumors (n = 37) based on their overall survival (P = 0.0479). In addition, serum proteins, such as c-met, that were top ranked at identifying bladder cancer patients were associated with pathological stage, tumor grade, and survival when validated by immunohistochemistry of tissue microarrays containing bladder tumors (n = 173). This study provides experimental evidence for the use of several integrated technologies strengthening the process of biomarker discovery. Serum protein profiles obtained by antibody arrays represent comprehensive means for bladder cancer diagnosis and clinical outcome stratification, which could potentially assist in selection of cancer patients who would benefit from early, individualized therapeutic intervention. Copyright © American Society for Investigative Pathology. |
| Keywords: | immunohistochemistry; epidermal growth factor; adult; cancer survival; controlled study; human tissue; protein expression; aged; aged, 80 and over; middle aged; protein array analysis; survival analysis; cancer staging; cancer diagnosis; cancer grading; sensitivity and specificity; prostate specific antigen; gene expression; gene expression profiling; tumor markers, biological; validation study; bladder cancer; protein p53; tumor marker; urinary bladder neoplasms; enzyme immunoassay; in situ hybridization; urothelium; tissue array analysis; dna microarray; antibodies; tissue microarray; enzyme-linked immunosorbent assay; serum; complementary dna; plasma protein; scatter factor receptor; cancer antibody; antibody array |
| Journal Title: | American Journal of Pathology |
| Volume: | 168 |
| Issue: | 1 |
| ISSN: | 0002-9440 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2006-06-01 |
| Start Page: | 93 |
| End Page: | 103 |
| Language: | English |
| DOI: | 10.2353/ajpath.2006.050601 |
| PUBMED: | 16400012 |
| PROVIDER: | scopus |
| PMCID: | PMC1592677 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 83" - "Export Date: 4 June 2012" - "CODEN: AJPAA" - "Source: Scopus" |
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