His239Arg SNP of HRAD9 is associated with lung adenocarcinoma Journal Article
| Authors: | Maniwa, Y.; Yoshimura, M.; Bermudez, V. P.; Okada, K.; Kanomata, M.; Ohbayashi, C.; Nishimura, Y.; Hayashi, Y.; Hurwitz, J.; Okita, Y. |
| Article Title: | His239Arg SNP of HRAD9 is associated with lung adenocarcinoma |
| Abstract: | BACKGROUND. It was previously reported that a functional human (h) Rad9 protein accumulated in the nuclei of nonsmall cell lung carcinoma (NSCLC) cells. Those experiments, however, did not examine whether the hRad9 gene was mutated in those cells. The sequence of the HRAD9 gene in NSCLC cells was investigated. METHODS. The sequence of the HRAD9 was examined in tumor and peripheral normal lung tissues obtained from 50 lung adenocarcinoma patients during surgery. The expression of its mRNA using reverse transcription polymerase chain reaction (RT-PCR) was also examined. RESULTS. No sequence alterations were detected in the HRAD9 gene, which was found to be normally transcribed in surgically resected lung carcinoma cells. However, in eight (16.0%) cases a single nucleotide polymorphism (SNP) was observed at the second position of codon 239 (His/Arg heterozygous variant) of the gene. This frequency was significantly higher than that found in the normal population. CONCLUSIONS. Whereas the capacity to produce a functional hRad9 protein was intact in lung adenocarcinoma cells, a nonsynonymous SNP of HRAD9 was detected that might be associated with the development of lung adenocarcinoma. © 2006 American Cancer Society. |
| Keywords: | clinical article; controlled study; human tissue; protein expression; gene mutation; gene sequence; human cell; single nucleotide polymorphism; polymorphism, single nucleotide; protein function; adenocarcinoma; cell cycle proteins; gene; disease association; reverse transcription polymerase chain reaction; lung non small cell cancer; carcinoma, non-small-cell lung; lung neoplasms; lung adenocarcinoma; reverse transcriptase polymerase chain reaction; heterozygosity; nucleotide sequence; dna mutational analysis; codon; arginine; histidine; nonsmall cell lung carcinoma; protein rad9; rad9; nonsynonymous single nucleotide polymorphism; cell cycle control; dna damage check-point pathway; hrad9 gene |
| Journal Title: | Cancer |
| Volume: | 106 |
| Issue: | 5 |
| ISSN: | 0008-543X |
| Publisher: | Wiley Blackwell |
| Date Published: | 2006-03-01 |
| Start Page: | 1117 |
| End Page: | 1122 |
| Language: | English |
| DOI: | 10.1002/cncr.21705 |
| PUBMED: | 16444745 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 11" - "Export Date: 4 June 2012" - "CODEN: CANCA" - "Source: Scopus" |
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