Enhancement of clonogenicity of human multiple myeloma by dendritic cells Journal Article
| Authors: | Kukreja, A.; Hutchinson, A.; Dhodapkar, K.; Mazumder, A.; Vesole, D.; Angitapalli, R.; Jagannath, S.; Dhodapkar, M. V. |
| Article Title: | Enhancement of clonogenicity of human multiple myeloma by dendritic cells |
| Abstract: | Infiltration by dendritic cells (DCs) is a common feature of most human tumors. Prior studies evaluating the interaction of DCs with tumors have focused largely on their immunologic properties (for review see Banchereau, J., and R.M. Steinman. 1998. Nature. 392:245-252). In this study, we show that the clonogenicity of several human tumor cell lines and primary tumor cells from myeloma patients is enhanced by their interactions with DCs. Myeloma cells cultured in the presence of DCs have an altered phenotype with an increased proportion of cells lacking terminal plasma cell differentiation marker CD138. DC-tumor interaction also leads to the up-regulation of B cell lymphoma 6 expression in myeloma cells. Effects of DCs on myeloma cells are inhibited by blockade of the receptor activator of NF-kB (RANK)-RANK ligand and B cell-activating factor-APRIL (a proliferation-inducing ligand)-mediated interactions. Together, these data suggest that tumor-DC interactions may directly impact the biology of human tumors, particularly multiple myeloma, and may be a target for therapeutic intervention. JEM © The Rockefeller University Press. |
| Keywords: | controlled study; protein expression; human cell; phenotype; multiple myeloma; bone marrow; cell growth; dendritic cell; cell differentiation; cancer cell culture; in vitro study; tumor cells, cultured; cell population; plasma cell; dendritic cells; membrane glycoproteins; upregulation; protein bcl 6; cell marker; cell interaction; osteoclast differentiation factor; clonogenesis; coculture techniques; syndecan 1; myeloma cell; tumor stem cell assay; proteoglycans; syndecan-1; b lymphocyte activation; april protein; syndecans |
| Journal Title: | Journal of Experimental Medicine |
| Volume: | 203 |
| Issue: | 8 |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2006-08-07 |
| Start Page: | 1859 |
| End Page: | 1865 |
| Language: | English |
| DOI: | 10.1084/jem.20052136 |
| PUBMED: | 16880256 |
| PROVIDER: | scopus |
| PMCID: | PMC2034506 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 51" - "Export Date: 4 June 2012" - "CODEN: JEMEA" - "Source: Scopus" |
