Fission yeast Mcm10p contains primase activity Journal Article
| Authors: | Fien, K.; Hurwitz, J. |
| Article Title: | Fission yeast Mcm10p contains primase activity |
| Abstract: | Although Mcm10p is a conserved essential component in eukaryotes required for both the initiation and elongation of DNA chains, its biochemical properties are unknown. Here, we report that the Schizosaccharomyces pombe fission yeast Mcm10protein contains primase activity. Primases are enzymes that synthesize RNA primers on single-stranded DNA templates that are extended by DNA polymerases. In keeping with this property, Mcm10p supported oligoribonucleotide synthesis of short RNA primers (preferentially initiating synthesis on a dT template) that were extended with dATP by Escherichia coli DNA polymerase I. The C terminus of Mcm10p synthesized RNA, but less efficiently than the full-length protein at low rNTP levels. Mcm10p homologs contain a C-terminal motif found in proteins that polymerize nucleotides. A point mutant within this motif of S. pombe Mcm10p was defective in primer synthesis in vitro, and this mutant failed to support growth in vivo, suggesting that the primase activity of Mcm10p may be essential for cell viability. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc. |
| Keywords: | controlled study; unclassified drug; nonhuman; dna polymerase; dna synthesis; protein function; protein motif; proteins; cell cycle proteins; cell viability; cell division; cell growth; carboxy terminal sequence; in vivo study; in vitro study; enzyme activity; rna; biosynthesis; dna; amino acid sequence; kinetics; rna synthesis; eukaryota; nucleotide sequence; escherichia coli; polymerization; peptide fragments; yeast; sequence homology; point mutation; biochemistry; mutagenesis; schizosaccharomyces; schizosaccharomyces pombe proteins; schizosaccharomyces pombe; nucleotides; dna directed dna polymerase beta; dna template; templates, genetic; dna primase; oligoribonucleotide synthesis; primase activity; rna primers; oligoribonucleotide; primer rna; protein mcm10; oligoribonucleotides |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 281 |
| Issue: | 31 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2006-08-04 |
| Start Page: | 22248 |
| End Page: | 22260 |
| Language: | English |
| DOI: | 10.1074/jbc.M512997200 |
| PUBMED: | 16720577 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 11" - "Export Date: 4 June 2012" - "CODEN: JBCHA" - "Source: Scopus" |
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