Novel mutations of epidermal growth factor receptor in localized prostate cancer Journal Article
| Authors: | Douglas, D. A.; Zhong, H.; Ro, J. Y.; Oddoux, C.; Berger, A. D.; Pincus, M. R.; Satagopan, J. M.; Gerald, W. L.; Scher, H. I.; Lee, P.; Osman, I. |
| Article Title: | Novel mutations of epidermal growth factor receptor in localized prostate cancer |
| Abstract: | We recently demonstrated that EGFR protein overexpression is more common in African American (AA) prostate cancer patients compared to Caucasian patients. We further examine EGFR dysregulation by determining EGFR mutation status in the tyrosine kinase (TK) domain in prostate cancer patients of different ethnicity. Normal and tumor DNA from 89 radical prostatectomy cases were studied for mutations in the EGFR TK domain using genomic DNA sequencing. We identified 4 novel missense mutations in exons 19, 20 and 21 of EGFR TK domain: 3 in Koreans and 1 in Caucasian but none in AA. We also identified 5 distinct synonymous DNA sequence changes, which did not alter the encoded amino acid, in exons 20 and 21 in 31/89 (35%) patients. Interestingly, these synonymous sequence changes were not observed in normal DNA in 7(23%) patients, indicating the presence of de novo somatic mutation to a new synonymous sequence. Our data reveal that EGFR missense mutation in the TK domain occurs in localized prostate cancer. Our data also demonstrate the presence of somatic mutation to a new synonymous sequence in a subset of patients. Larger population-based studies are required to define the association between EGFR mutations and the ethnic background of patients. |
| Keywords: | immunohistochemistry; aged; middle aged; case control study; genetics; missense mutation; mutation; case-control studies; mutation, missense; polymerase chain reaction; epidermal growth factor receptor; receptor, epidermal growth factor; protein tyrosine kinase; prostatic neoplasms; prostate; nucleotide sequence; prostate tumor; protein-tyrosine kinases; dna mutational analysis; egfr; tumor; neoplasia; adencarcinoma |
| Journal Title: | Frontiers in Bioscience |
| Volume: | 11 |
| Issue: | Suppl.2 |
| ISSN: | 1093-9946 |
| Publisher: | Frontiers in Bioscience |
| Date Published: | 2006-09-01 |
| Start Page: | 2518 |
| End Page: | 2525 |
| Language: | English |
| DOI: | 10.2741/1986 |
| PUBMED: | 16720329 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 16" - "Export Date: 4 June 2012" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work