Array comparative genomic hybridization reveals genomic copy number changes associated with outcome in diffuse large B-cell lymphomas Journal Article

   

Authors:	Chen, W.; Houldsworth, J.; Olshen, A. B.; Nanjangud, G.; Chaganti, S.; Venkatraman, E. S.; Halaas, J.; Teruya-Feldstein, J.; Zelenetz, A. D.; Chaganti, R. S. K.
Article Title:	Array comparative genomic hybridization reveals genomic copy number changes associated with outcome in diffuse large B-cell lymphomas
Abstract:	To identify, in high-resolution regions of DNA, the copy number changes associated with outcome in patients with diffuse large B-cell lymphoma (DLBCL), a disease with an approximately 50% mortality rate, we performed array comparative genomic hybridization (array-CGH) on specimens from 64 patients with newly diagnosed DLBCL treated with anthracycline-based chemotherapy. For the entire cohort, 55 commonly gained/lost regions, ranging in size from less than 1 Mbp to entire chromosomes, were identified using 1- to 2-Mbp and 2- to 4-Mbp resolution BAC arrays. Copy number changes of 9 minimal regions significantly correlated with overall survival, of which 6 were 10 Mbp or smaller. On multivariate analysis, loss of chromosomes 2 (2.4-4.1 Mbp) and 16 (33.8-35.6 Mbp) were found to be prognostic indicators of poor survival, independent of clinical features routinely used to predict outcome. Loss of chromosome 1 (78.2-79.1 Mbp) was predictive of good outcome. For a subset of 55 specimens classified according to cell-of-origin expression signature subtype, gain of chromosome 12 (45.4-53.8 Mbp) was found to be significantly associated with the germinal center B-cell-likeDLBCLsubtype. Overall, array-CGH identified relatively small genomic regions associated with outcome, which, along with follow-up expression studies, may reveal target genes important in DLBCL clinical behavior. © 2006 by The American Society of Hematology.
Keywords:	cancer chemotherapy; cancer survival; controlled study; treatment outcome; survival rate; major clinical study; clinical feature; disease classification; chromosome 12; in situ hybridization, fluorescence; cohort analysis; prediction; b cell lymphoma; germinal center; lymphoma, b-cell; correlation analysis; dna; genomics; multivariate analysis; large cell lymphoma; gene dosage; nucleic acid hybridization; anthracycline; anthracyclines; chromosome analysis; chromosome loss; chromosomes, human; comparative genomic hybridization; chromosome 2; lymphoma, large-cell, diffuse
Journal Title:	Blood
Volume:	107
Issue:	6
ISSN:	0006-4971
Publisher:	American Society of Hematology
Date Published:	2006-03-15
Start Page:	2477
End Page:	2485
Language:	English
DOI:	10.1182/blood-2005-07-2950
PUBMED:	16317097
PROVIDER:	scopus
PMCID:	PMC1895737
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-33644771109&partnerID=40&md5=61dc2fe93b1b7a3279af2f0dad346d2f
Notes:	--- - "Cited By (since 1996): 51" - "Export Date: 4 June 2012" - "CODEN: BLOOA" - "Source: Scopus"

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