Immunoreactivity assay for α-particle emitting monoclonal antibody constructs Journal Article
| Authors: | Bonavia, A. S.; McDevitt, M. R.; Curcio, M. J.; Scheinberg, D. A. |
| Article Title: | Immunoreactivity assay for α-particle emitting monoclonal antibody constructs |
| Abstract: | Clinical trials using α-particle radiolabeled antibodies require a rapid and reproducible assay of the immunoreactivity of drugs. While live cell assays are typically used to determine the immunoreactive drug fraction, a fixed cell assay may replace the traditional live cell assay and offer the advantages of rapidity, easy availability and consistency for qualifying drugs for preclinical or clinical studies. We have identified optimal cell fixation and immunoreactivity assay conditions and have validated them by performing the fixed-cell assay in clinical trials. © 2005 Elsevier Ltd. All rights reserved. |
| Keywords: | controlled study; human cell; nonhuman; animal cell; mouse; animals; mice; cell line; cell assay; immunoreactivity; monoclonal antibody; antibodies, monoclonal; isotope labeling; bioassay; radioisotopes; immunoglobulin g1 antibody; cells; medical applications; alpha radiation; alpha particles; drug products; actinium 225; bismuth 213; ac-225; α-particles; bi-213; immunoreactivity assay; particulate emissions |
| Journal Title: | Applied Radiation and Isotopes |
| Volume: | 64 |
| Issue: | 4 |
| ISSN: | 0969-8043 |
| Publisher: | Pergamon-Elsevier Science Ltd |
| Date Published: | 2006-04-01 |
| Start Page: | 470 |
| End Page: | 474 |
| Language: | English |
| DOI: | 10.1016/j.apradiso.2005.11.004 |
| PUBMED: | 16352433 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 4 June 2012" - "CODEN: ARISE" - "Source: Scopus" |
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499Scheinberg -
28Curcio -
1Bonavia
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