Virally-directed fluorescent imaging (VFI) can facilitate endoscopic staging Journal Article
| Authors: | Adusumilli, P. S.; Eisenberg, D. P.; Stiles, B. M.; Hendershott, K. J.; Stanziale, S. F.; Chan, M. K.; Hezel, M.; Huq, R.; Rusch, V. W.; Fong, Y. |
| Article Title: | Virally-directed fluorescent imaging (VFI) can facilitate endoscopic staging |
| Abstract: | Background: Replication-competent, tumor specific herpes simplex virus NV1066 expresses green fluorescent protein (GFP) in infected cancer cells. We sought to determine the feasibility of GFP-guided imaging technology in the intraoperative detection of small tumor nodules. Methods: Human cancer cell lines were infected with NV1066 at multiplicities of infection of 0.01, 0.1 and 1. Cancer cell specific infectivity, vector spread and GFP signal intensity were measured by flow cytometry and time-lapse digital imaging (in vitro); and by use of a stereomicroscope and endoscope equipped with a fluorescent filter (in vivo). Results: NV1066 infected all cancer cell lines and expressed GFP at all MOIs. GFP signal was significantly higher than the autofluorescence of normal cells. One single dose of NV1066 spread within and across body cavities and selectively infected tumor nodules sparing normal tissue. Tumor nodules undetectable by conventional thoracoscopy and laparoscopy were identified by GFP fluorescence. Conclusion: Virally-directed fluorescent imaging (VFI) is a real-time novel molecular imaging technology that has the potential to enhance the intraoperative detection of endoluminal or endocavitary tumor nodules. © Springer Science+Business Media, Inc. 2006. |
| Keywords: | controlled study; human cell; nonhuman; pancreas cancer; cancer staging; flow cytometry; neoplasm staging; laparoscopy; diagnostic accuracy; sensitivity analysis; neoplasms; animal cell; mouse; animals; mice; animal tissue; cell death; metastasis; peritoneum cancer; peritoneal neoplasms; fluorescence; green fluorescent protein; animal experiment; animal model; lung cancer; herpes simplex; cancer cell culture; in vitro study; molecular imaging; cell line, tumor; diagnostic imaging; time factors; feasibility study; feasibility studies; colon cancer; measurement; carcinoma; gene therapy; oncolytic virus; simplexvirus; oncolytic viruses; stomach cancer; green fluorescent proteins; microscopy, fluorescence; liver cancer; virus infection; virus replication; esophagus cancer; endoscopy; herpes simplex virus; gallbladder cancer; signal detection; pleural neoplasms; thoracoscopy; tumor diagnosis; expression vector; pleura cancer; digital imaging; stereomicroscopy; microscope; endoscope; luminescent agents; fluorescent detection; fluorescent laparoscopy; fluorescent thoracoscopy; virally directed fluorescent imaging |
| Journal Title: | Surgical Endoscopy |
| Volume: | 20 |
| Issue: | 4 |
| ISSN: | 0930-2794 |
| Publisher: | Springer |
| Date Published: | 2006-04-01 |
| Start Page: | 628 |
| End Page: | 635 |
| Language: | English |
| DOI: | 10.1007/s00464-005-0259-6 |
| PUBMED: | 16446989 |
| PROVIDER: | scopus |
| PMCID: | PMC1435379 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 4" - "Export Date: 4 June 2012" - "CODEN: SUREE" - "Source: Scopus" |
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