Heterogeneous nuclear ribonucleoprotein L is a subunit of human KMT3a/Set2 complex required for H3 Lys-36 trimethylation activity in vivo Journal Article
| Authors: | Yuan, W.; Xie, J. W.; Long, C. Z.; Erdjument-Bromage, H.; Ding, X. J.; Zheng, Y.; Tempst, P.; Chen, S.; Zhu, B.; Reinberg, D. |
| Article Title: | Heterogeneous nuclear ribonucleoprotein L is a subunit of human KMT3a/Set2 complex required for H3 Lys-36 trimethylation activity in vivo |
| Abstract: | The presence of histone H3 lysine 36 methylation (H3K36me) correlates with actively transcribed genes. In yeast, histone H3K36me mediated by KMT3 (also known as Set2) recruits a histone deacetylase complex, Rpd3s, to ensure the fidelity of transcription initiation. We report the purification of human KMT3a (also known as HYPB or hSet2) complex and the identification of a novel, higher eukaryotic specific subunit, heterogeneous nuclear ribonucleoprotein L (HnRNP-L). Interestingly, although KMT3a has intrinsic activity in vitro, HnRNP-L is essential in vivo. Moreover, KMT3a generates mono-, di-, and trimethylated products in vitro, but RNA interference against KMT3a or HnRNP-L down-regulates exclusively the H3K36me3 mark in vivo. |
| Keywords: | chromatin; histone h3; saccharomyces-cerevisiae; rna-polymerase-ii; gene-expression; messenger-rnas; hnrnp-l; transcriptional elongation; modifications; h3k36 methylation; set2 |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 284 |
| Issue: | 23 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2009-06-05 |
| Start Page: | 15701 |
| End Page: | 15707 |
| Language: | English |
| ACCESSION: | ISI:000266501000039 |
| DOI: | 10.1074/jbc.M808431200 |
| PROVIDER: | wos |
| PMCID: | PMC2708867 |
| PUBMED: | 19332550 |
| Notes: | --- - Article - "Source: Wos" |
