Regulation of adipogenesis by lymphatic fluid stasis: Part I. Adipogenesis, fibrosis, and inflammation Journal Article


Authors: Zampell, J. C.; Aschen, S.; Weitman, E. S.; Yan, A.; Elhadad, S.; De Brot, M.; Mehrara, B. J.
Article Title: Regulation of adipogenesis by lymphatic fluid stasis: Part I. Adipogenesis, fibrosis, and inflammation
Abstract: Background: Although fat deposition is a defining clinical characteristic of lymphedema, the cellular mechanisms that regulate this response remain unknown. The goals of this two-part study were to determine the effect of lymphatic fluid stasis on adipogenesis and inflammation (part I) and how these changes regulate the temporal and spatial expression of fat differentiation genes (part II). Methods: Adult female mice underwent tail lymphatic ablation and were euthanized 6 weeks after surgery (n = 20). Fat deposition, fibrosis, and inflammation were then analyzed in the regions of the tail exposed to lymphatic fluid stasis as compared with normal lymphatic flow. Results: Lymphatic fluid stasis in the tail resulted in significant subcutaneous fat deposition, with a 2-fold increase in fat thickness (p < 0.01). In addition, lymphatic stasis was associated with subcutaneous fat fibrosis and collagen deposition. Adipogenesis in response to lymphatic fluid stasis was associated with a marked mononuclear cell inflammatory response (5-fold increase in CD45 cells; p < 0.001). In addition, the authors noted a significant increase in the number of monocytes/macrophages as identified by F4/80 immunohistochemistry (p < 0.001). Conclusions: The mouse-tail model has pathologic findings that are similar to clinical lymphedema, including fat deposition, fibrosis, and inflammation. Adipogenesis in response to lymphatic fluid stasis closely resembles this process in obesity. This model therefore provides an excellent means with which to study the molecular mechanisms that regulate the pathophysiology of lymphedema. © 2012 by the American Society of Plastic Surgeons.
Journal Title: Plastic and Reconstructive Surgery
Volume: 129
Issue: 4
ISSN: 0032-1052
Publisher: Lippincott Williams & Wilkins  
Date Published: 2012-04-01
Start Page: 825
End Page: 834
Language: English
DOI: 10.1097/PRS.0b013e3182450b2d
PROVIDER: scopus
PUBMED: 22456354
PMCID: PMC3433726
DOI/URL:
Notes: --- - "Export Date: 1 May 2012" - "CODEN: PRSUA" - "Source: Scopus"
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MSK Authors
  1. Sonia Elhadad
    10 Elhadad
  2. Babak Mehrara
    293 Mehrara
  3. Jamie Christine Zampell
    29 Zampell
  4. Alan Yan
    20 Yan
  5. Evan Scott Weitman
    18 Weitman
  6. Seth Aschen
    13 Aschen