A secretion of the mollusc Cryptomphalus aspersa promotes proliferation, migration and survival of keratinocytes and dermal fibroblasts in vitro Journal Article

Authors: Iglesias-De La Cruz, M. C.; Sanz-Rodriguez, F.; Zamarrón, A.; Reyes, E.; Carrasco, E.; Gonzalez, S.; Juarranz, A.
Article Title: A secretion of the mollusc Cryptomphalus aspersa promotes proliferation, migration and survival of keratinocytes and dermal fibroblasts in vitro
Abstract: Synopsis Regenerative properties of skin decrease with age, and thus, the search for substances that minimize cutaneous ageing has increased in the last few years. The secretion of the mollusc Cryptomphalus Aspersa (SCA) is a natural product that bears regenerative properties when applied topically. The purpose of this work is to study the in vitro effects of SCA on cell proliferation and migration, as well as on cell-cell (E-cadherin and β-catenin) and cell-substrate (vinculin and β1-integrin) adhesion proteins expression, using a human keratinocyte cell line (HaCaT cells) and primary dermal fibroblasts (HF). We tested the effects of SCA on cell proliferation using a colorimetric assay. In addition, SCA-induced changes on cell migration were studied by wound-healing assays. Besides, Western blot and immunofluorescence microscopy were carried out to test the expression of different cell adhesion proteins. We found that SCA promotes proliferation and migration of HaCaT cells in a time- and dose-dependent manner. Moreover, treatment with SCA increases the migratory behaviour and the expression of adhesion molecules in both HaCaT and HF. Finally, SCA also improves cell survival and promotes phosphorylation of FAK and nuclear localization of β-catenin. These results shed light on the molecular mechanisms underlying the regenerative properties of SCA, based on its promoting effect on skin cell migration, proliferation and survival. Moreover, these results support future clinical uses of SCA in the regeneration of wounded tissues. © 2011 Society of Cosmetic Scientists and the Société Française de Cosmétologie.
Keywords: controlled study; protein expression; unclassified drug; human cell; dose response; nonhuman; protein localization; cell proliferation; cell survival; in vitro study; drug effect; cell assay; uvomorulin; keratinocyte; wound healing; enzyme phosphorylation; drug mechanism; western blotting; cell migration; fibroblasts; cellular distribution; cell regeneration; proliferation; natural product; beta catenin; keratinocytes; cytoskeleton; focal adhesion kinase; drug isolation; immunofluorescence microscopy; skin fibroblast; cell adhesion molecule; cell adhesion components; cell strain; cutaneous parameters; beta1 integrin; cryptomphalus aspersa; mollusc; cryptomphalus aspersa secretion; vinculin; colorimetry
Journal Title: International Journal of Cosmetic Science
Volume: 34
Issue: 2
ISSN: 0142-5463
Publisher: Wiley Blackwell  
Date Published: 2012-04-01
Start Page: 183
End Page: 189
Language: English
DOI: 10.1111/j.1468-2494.2011.00699.x
PROVIDER: scopus
PUBMED: 22171745
Notes: --- - "Export Date: 1 May 2012" - "CODEN: IJCMD" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Salvador Gonzalez Rodriguez
    203 Rodriguez