CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA Journal Article
| Authors: | Shamay, M.; Hand, N.; Lemas, M. V.; Koon, H. B.; Krown, S. E.; Wrangle, J.; Desai, P.; Ramos, J. C.; Ambinder, R. F. |
| Article Title: | CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA |
| Abstract: | We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue. © The Author 2012. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. |
| Journal Title: | Journal of Infectious Diseases |
| Volume: | 205 |
| Issue: | 7 |
| ISSN: | 0022-1899 |
| Publisher: | Oxford University Press |
| Date Published: | 2012-04-01 |
| Start Page: | 1095 |
| End Page: | 1099 |
| Language: | English |
| DOI: | 10.1093/infdis/jis032 |
| PROVIDER: | scopus |
| PMCID: | PMC3295606 |
| PUBMED: | 22357696 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 2 April 2012" - "CODEN: JIDIA" - "Source: Scopus" |
