Interleukin 23 production by intestinal CD103(+)CD11b(+) dendritic cells in response to bacterial flagellin enhances mucosal innate immune defense Journal Article

   

Authors:	Kinnebrew, M. A.; Buffie, C. G.; Diehl, G. E.; Zenewicz, L. A.; Leiner, I.; Hohl, T. M.; Flavell, R. A.; Littman, D. R.; Pamer, E. G.
Article Title:	Interleukin 23 production by intestinal CD103(+)CD11b(+) dendritic cells in response to bacterial flagellin enhances mucosal innate immune defense
Abstract:	Microbial penetration of the intestinal epithelial barrier triggers inflammatory responses that include induction of the bactericidal C-type lectin RegIIIγ. Systemic administration of flagellin, a bacterial protein that stimulates Toll-like receptor 5 (TLR5), induces epithelial expression of RegIIIγ and protects mice from intestinal colonization with antibiotic-resistant bacteria. Flagellin-induced RegIIIγ expression is IL-22 dependent, but how TLR signaling leads to IL-22 expression is incompletely defined. By using conditional depletion of lamina propria dendritic cell (LPDC) subsets, we demonstrated that CD103 +CD11b + LPDCs, but not monocyte-derived CD103 -CD11b + LPDCs, expressed high amounts of IL-23 after bacterial flagellin administration and drove IL-22-dependent RegIIIγ production. Maximal expression of IL-23 subunits IL-23p19 and IL-12p40 occurred within 60 min of exposure to flagellin. IL-23 subsequently induced a burst of IL-22 followed by sustained RegIIIγ expression. Thus, CD103 +CD11b + LPDCs, in addition to promoting long-term tolerance to ingested antigens, also rapidly produce IL-23 in response to detection of flagellin in the lamina propria. © 2012 Elsevier Inc.
Keywords:	controlled study; protein expression; unclassified drug; nonhuman; protein analysis; animal cell; mouse; dendritic cell; cd11b antigen; cytokine production; innate immunity; interleukin 12p40; flagellin; lectin; interleukin 23; cd103 antigen; lamina propria; interleukin 23p19; reg iii gamma protein
Journal Title:	Immunity
Volume:	36
Issue:	2
ISSN:	1074-7613
Publisher:	Cell Press
Date Published:	2012-02-24
Start Page:	276
End Page:	287
Language:	English
DOI:	10.1016/j.immuni.2011.12.011
PROVIDER:	scopus
PMCID:	PMC3288454
PUBMED:	22306017
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84857444876&partnerID=40&md5=c959ff0dfaec9684de4288da7fdf5bab
Notes:	--- - "Export Date: 2 April 2012" - "CODEN: IUNIE" - "Source: Scopus"

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