Exploiting Drosophila genetics to understand MicroRNA function and regulation Journal Article

   

Authors:	Dai, Q.; Smibert, P.; Lai, E. C.
Article Title:	Exploiting Drosophila genetics to understand MicroRNA function and regulation
Abstract:	Although a great deal is known about the identity, biogenesis, and targeting capacity of microRNAs (miRNAs) in animal cells, far less is known about their functional requirements at the organismal level. Much remains to be understood about the necessity of miRNAs for overt phenotypes, the identity of critical miRNA targets, and the control of miRNA transcription. In this review, we provide an overview of genetic strategies to study miRNAs in the Drosophila system, including loss- and gain-of-function techniques, genetic interaction strategies, and transgenic reporters of miRNA expression and activity. As we illustrate the usage of these techniques in intact Drosophila, we see certain recurrent themes for miRNA functions, including energy homeostasis, apoptosis suppression, growth control, and regulation of core cell signaling pathways. Overall, we hope that this exposition of Drosophila genetic techniques, well known to the legions of fly geneticists and used to study all genes, can inform the general miRNA community that focuses on other biochemical, molecular, computational, and structural avenues. Clearly, it is the combination of these myriad techniques that has accelerated miRNA research to its extraordinary pace. © 2012 Elsevier Inc.
Keywords:	microrna; drosophila; knockout; sensor; epistasis; ep element; gal4-uas system; sponge
Journal Title:	Current Topics in Developmental Biology
Volume:	99
ISSN:	0070-2153
Publisher:	Academic Press, Elsevier Inc
Date Published:	2012-01-01
Start Page:	201
End Page:	235
Language:	English
DOI:	10.1016/b978-0-12-387038-4.00008-2
PROVIDER:	scopus
PUBMED:	22365740
PMCID:	PMC4505732
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84857412362&partnerID=40&md5=92f0a6baf0cd19fe98d97e9a281f7ba2
Notes:	--- Chapter 8 in "MicroRNAs in Development" (ISBN: 978-0-12-387038-4) - "Export Date: 2 April 2012" - "Source: Scopus"

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