Colon cancer associated transcript-1: A novel RNA expressed in malignant and pre-malignant human tissues Journal Article


Authors: Nissan, A.; Stojadinovic, A.; Mitrani-Rosenbaum, S.; Halle, D.; Grinbaum, R.; Roistacher, M.; Bochem, A.; Dayanc, B. E.; Ritter, G.; Gomceli, I.; Bostanci, E. B.; Akoglu, M.; Chen, Y. T.; Old, L. J.; Gure, A. O.
Article Title: Colon cancer associated transcript-1: A novel RNA expressed in malignant and pre-malignant human tissues
Abstract: Early detection of colorectal cancer (CRC) is currently based on fecal occult blood testing (FOBT) and colonoscopy, both which can significantly reduce CRC-related mortality. However, FOBT has low-sensitivity and specificity, whereas colonoscopy is labor- and cost-intensive. Therefore, the discovery of novel biomarkers that can be used for improved CRC screening, diagnosis, staging and as targets for novel therapies is of utmost importance. To identify novel CRC biomarkers we utilized representational difference analysis (RDA) and characterized a colon cancer associated transcript (CCAT1), demonstrating consistently strong expression in adenocarcinoma of the colon, while being largely undetectable in normal human tissues (p < 000.1). CCAT1 levels in CRC are on average 235-fold higher than those found in normal mucosa. Importantly, CCAT1 is strongly expressed in tissues representing the early phase of tumorigenesis: in adenomatous polyps and in tumor-proximal colonic epithelium, as well as in later stages of the disease (liver metastasis, for example). In CRC-associated lymph nodes, CCAT1 overexpression is detectable in all H&E positive, and 40.0% of H&E and immunohistochemistry negative lymph nodes, suggesting very high sensitivity. CCAT1 is also overexpressed in 40.0% of peripheral blood samples of patients with CRC but not in healthy controls. CCAT1 is therefore a highly specific and readily detectable marker for CRC and tumor-associated tissues. Copyright © 2011 UICC.
Keywords: colorectal cancer; biomarkers; minimal residual disease; non-coding rna
Journal Title: International Journal of Cancer
Volume: 130
Issue: 7
ISSN: 0020-7136
Publisher: John Wiley & Sons  
Date Published: 2012-04-01
Start Page: 1598
End Page: 1606
Language: English
DOI: 10.1002/ijc.26170
PROVIDER: scopus
PUBMED: 21547902
DOI/URL:
Notes: --- - "Export Date: 1 March 2012" - "CODEN: IJCNA" - "Source: Scopus"
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
  1. Gerd Ritter
    166 Ritter
  2. Lloyd J Old
    593 Old