Expression of Helios in peripherally induced Foxp3(+) regulatory T cells Journal Article
| Authors: | Gottschalk, R. A.; Corse, E.; Allison, J. P. |
| Article Title: | Expression of Helios in peripherally induced Foxp3(+) regulatory T cells |
| Abstract: | The transcription factor Helios has been reported to be a marker of regulatory T cells (Treg) of thymic origin, distinguishing them from Treg induced in the periphery (iTreg). In this study, we demonstrate Helios expression in Foxp3 + iTreg, both in vitro and in vivo. Following i.v. peptide injection, in vivo Helios expression in adoptively transferred TCR transgenic T cells was more rapid than Foxp3 induction but less stable at later time points without a second injection of peptide. Our in vitro data suggest that APC influence Helios expression in a manner distinct from stimuli required for Foxp3 induction. Thus, Helios expression in iTreg may reflect the context of stimulation during Foxp3 induction. In summary, the robust Helios expression we observe in iTreg precludes its use as a marker of thymic Treg. Copyright © 2012 by The American Association of Immunologists, Inc. |
| Keywords: | signal transduction; controlled study; protein expression; unclassified drug; nonhuman; protein localization; transcription factor foxp3; animal cell; mouse; interleukin 2; transforming growth factor beta; animal experiment; animal model; protein stability; transcription factor; in vivo study; in vitro study; t lymphocyte receptor; regulatory t lymphocyte; cellular distribution; adoptive transfer; upregulation; protein determination; antigen presenting cell; t lymphocyte activation; cd45 antigen; peptide analysis; cell kinetics; transcription factor helios; lymphocyte transfer |
| Journal Title: | Journal of Immunology |
| Volume: | 188 |
| Issue: | 3 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 2012-02-01 |
| Start Page: | 976 |
| End Page: | 980 |
| Language: | English |
| DOI: | 10.4049/jimmunol.1102964 |
| PROVIDER: | scopus |
| PUBMED: | 22198953 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 March 2012" - "CODEN: JOIMA" - "Source: Scopus" |
