A 20-color 21-antigen flow cytometric assay for disease monitoring of T-cell lymphoblastic leukemia Journal Article


Authors: Gao, Q.; Zhang, J.; Rozenova, K.; Sun, X.; Burke, A.; Miu, O.; Nguyen, N.; Zhang, S. J.; Roshal, M.
Article Title: A 20-color 21-antigen flow cytometric assay for disease monitoring of T-cell lymphoblastic leukemia
Abstract: T-lineage acute lymphoblastic leukemia (T-ALL) is an aggressive neoplasm of immature T cells. Flow cytometry plays a critical role in the diagnosis and management of the disease. It is used to establish the abnormal immature T-cell phenotype and to distinguish the early T-cell precursor (ETP)-ALL from more mature types at diagnosis. The evaluation of mediastinal disease is often complicated by the difficulty of the phenotypic distinction between the normal thymic precursors and the abnormal T lymphoblasts. Follow-up measurements of minimal/measurable residual disease (MRD) are critical for therapy decision-making and prognostication. In the MRD setting, flow cytometry requires a high degree of analytical expertise and assessment of numerous antigens. To address the diagnostic and monitoring challenges, we developed a single-tube 21-antigen assessment with simplified analysis. The assay distinguishes between normal thymic precursors and T lymphoblasts in tissue samples, enables evaluation of ETP versus non-ETP phenotypes, and allows for MRD assessment below 0.01% robust to antigenic changes. © 2025 The Author(s). Cytometry Part B: Clinical Cytometry published by Wiley Periodicals LLC on behalf of International Clinical Cytometry Society.
Keywords: t-all; thymocytes; mrd; assay validation; llod; lloq; etp
Journal Title: Cytometry Part B—Clinical Cytometry
Volume: 108
Issue: 4
ISSN: 1552-4949
Publisher: Wiley Blackwell  
Date Published: 2025-07-01
Start Page: 299
End Page: 311
Language: English
DOI: 10.1002/cyto.b.22242
PUBMED: 40605707
PROVIDER: scopus
DOI/URL:
Notes: Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- MSK corresponding author is Mikhail Roshal -- Source: Scopus
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MSK Authors
  1. Mikhail Roshal
    236 Roshal
  2. Qi   Gao
    69 Gao
  3. Nghia Trong Nguyen
    6 Nguyen
  4. Jing-Ping Zhang
    7 Zhang
  5. Xiaotian Sun
    10 Sun
  6. Amanda Phyllis Burke
    3 Burke
  7. Shu Jie Zhang
    2 Zhang
  8. Man Hing (Olivia) Miu
    2 Miu