Donor embryonic stem cells displace host cells of 8-cell-stage chimeras to the extra-embryonic lineages by spatial crowding and FGF4 signalling Journal Article

   

Authors:	Strawbridge, S. E.; Schrattel, A. K.; Humphreys, P.; Jones, K. A.; Artus, J.; Hadjantonakis, A. K.; Fletcher, A. G.; Nichols, J.
Article Title:	Donor embryonic stem cells displace host cells of 8-cell-stage chimeras to the extra-embryonic lineages by spatial crowding and FGF4 signalling
Abstract:	Following mouse embryo compaction, outer cells become trophectoderm, while inner cells form the inner cell mass (ICM), later differentiating into primitive endoderm and epiblast during blastocyst formation. Trophectoderm specification is driven by position-governed polarisation, while primitive endoderm specification is positively regulated by FGF4 signalling from the unspecified ICM and epiblast. When injected into an 8-cell-stage morula, embryonic stem cells (ESCs; derived from pre-implantation epiblast cells in vitro) can exclude host cells from the epiblast, leading to mice derived entirely from these cells. While evidence suggests roles for ESC-produced FGF4 and physical crowding in host cell displacement from the ICM, the interplay between these possible mechanisms has yet to be dissected, in part due to the lack of studies using Fgf4−/− ESCs. Here, we combine chimera titration assays with mathematical modelling to study these mechanisms of host cell displacement. Both Fgf4+/+ and Fgf4−/− ESCs displaced host cells from the epiblast, while only Fgf4−/− ESC-injected embryos reduced primitive endoderm and increased trophectoderm, indicating sequential exclusion by displacement crowding followed by FGF4 signalling. © 2025. Published by The Company of Biologists.
Keywords:	signal transduction; genetics; nonhuman; animal cell; mouse; animal; cytology; metabolism; animals; mice; animal tissue; embryo; embryonic stem cell; cell differentiation; in vitro study; cell lineage; chimera; embryonic stem cells; embryo, mammalian; upregulation; host cell; endoderm; mouse embryo; blastocyst; epiblast; primitive endoderm; inner cell mass; ectoderm; germ layer; morula; titrimetry; bayesian inference; germ layers; trophectoderm; fibroblast growth factor 4; mammalian embryo; mouse embryonic stem cells; article; mouse embryonic stem cell; mathematical modelling; fgf4 protein, mouse
Journal Title:	Development
Volume:	152
Issue:	12
ISSN:	0950-1991
Publisher:	Company of Biologists
Date Published:	2025-06-01
Start Page:	dev204518
Language:	English
DOI:	10.1242/dev.204518
PUBMED:	40446208
PROVIDER:	scopus
PMCID:	PMC12273629
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-105009657189&doi=10.1242%2fdev.204518&partnerID=40&md5=b3e1f913e6db1534c30718761630b30b
Notes:	Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/227236