A Novel Method for Culturing Telencephalic Neurons in Axolotls Journal Article
| Authors: | Bostan, S.; Serdengeçti, S.; Bayat, F. K.; Bay, S.; Sezer, A.; Ayşit, N.; Öztürk, G. |
| Article Title: | A Novel Method for Culturing Telencephalic Neurons in Axolotls |
| Abstract: | The axolotl (Ambystoma mexicanum), a neotenic salamander with remarkable regenerative capabilities, serves as a key model for studying nervous system regeneration. Despite its potential, the cellular and molecular mechanisms underlying this regenerative capacity remain poorly understood, partly due to the lack of reliable in vitro models for axolotl neural cells. In this study, we developed a novel protocol for primary cultures of adult axolotl telencephalon/pallium, enabling the maintenance of viable and functionally active neural cells. Using calcium imaging and immunocytochemistry, we demonstrated the presence of neuronal and glial markers, synaptic connections, and spontaneous calcium activity, highlighting the functional integrity of the cultured cells. Our findings reveal that these cultures can be maintained in both serum and serum-free conditions, with neurons exhibiting robust neurite outgrowth and responsiveness to injury. This protocol addresses a critical gap in axolotl research by providing a controlled in vitro system to study neurogenesis and regeneration. By offering insights into the regenerative mechanisms of axolotl neurons, this work lays the foundation for comparative studies with mammalian systems, potentially informing therapeutic strategies for neurodegenerative diseases and CNS injuries in humans. © 2025 The Author(s). The Journal of Comparative Neurology published by Wiley Periodicals LLC. |
| Keywords: | adult; controlled study; nonhuman; animal cell; animal tissue; cell viability; cell function; brain cortex; calcium; in vitro study; regeneration; blood sampling; immunocytochemistry; mammal; glia cell; nerve cell culture; nervous system development; nerve cell; degenerative disease; synapse; central nervous system disease; telencephalon; brain tissue; neurite outgrowth; human; article; brain regeneration; axolotl; primary neuron culture; ambystoma; telencephalic neuron |
| Journal Title: | Journal of Comparative Neurology |
| Volume: | 533 |
| Issue: | 6 |
| ISSN: | 0021-9967 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2025-01-01 |
| Start Page: | e70066 |
| Language: | English |
| DOI: | 10.1002/cne.70066 |
| PROVIDER: | scopus |
| PMCID: | PMC12177116 |
| PUBMED: | 40533890 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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