Synapse - Peritoneal washings analysis in endometrial cancer: Comparison of somatic mutation detection with panel sequencing and traditional cytology

Peritoneal washings analysis in endometrial cancer: Comparison of somatic mutation detection with panel sequencing and traditional cytology Journal Article

Authors:	Byrne, M. E.; Selenica, P.; Dessources, K.; Da Cruz Paula, A.; Gordhandas, S.; Wu, M.; Pareja, F.; Roche, K. L.; Mueller, J. J.; Sonoda, Y.; Abu-Rustum, N. R.; Weigelt, B.
Article Title:	Peritoneal washings analysis in endometrial cancer: Comparison of somatic mutation detection with panel sequencing and traditional cytology
Abstract:	Objectives: The prognostic significance of positive pelvic washings in endometrial cancer (EC) remains unknown, and little data exist regarding washings as a source of genetic information in relation to a patient's tumor. We sought to assess the feasibility of identifying EC mutations in peritoneal washings. Methods: Peritoneal washings from 21 biopsy-confirmed newly diagnosed patients with EC across disease stages between 09/2018 and 07/2019 were collected. Peritoneal washings, primary EC, and normal DNA samples were subjected to next-generation sequencing targeting 468 cancer-related genes. Sequencing results were compared to cytological analysis. Results: For the 21 EC cases included, cytology found 8 (38 %) of the peritoneal washings as positive, 7 (33 %) as negative, and 6 (29 %) as suspicious or rare-atypical cells. Based on molecular analysis, tumor mutations (TMs) were detected in 18/21 (86 %) of peritoneal washings. Overall, 11/21 (52 %) samples demonstrated concordant results between cytologic and molecular analysis, and all positive cytologic results were confirmed with molecular analysis. However, of cases with negative or suspicious cytology results, 77 % (10/13) were found to have TMs in washings. Five patients with negative cytology were positive on molecular analysis (5/7, 71 %), and 5 patients with suspicious washings demonstrated TMs (5/6, 83 %). Of the 10 EC patients who developed recurrences, regardless of stage, 5/10 (50 %) patients had positive cytology, whereas 9/10 (90 %) had TMs based on molecular analysis. Conclusions: Mutational analysis of peritoneal washings using panel sequencing in EC is feasible. A substantial subset of patients with cytology-negative or suspicious washings had TMs detected. © 2025 Elsevier Inc.
Keywords:	adult; clinical article; controlled study; human tissue; aged; primary tumor; somatic mutation; cancer recurrence; cancer patient; cancer staging; endometrioid carcinoma; endometrium cancer; cytology; peritoneum lavage; tumor biopsy; feasibility study; cancer cytodiagnosis; early cancer; microsatellite instability; dna extraction; endometrial carcinoma; endometrium biopsy; mutation detection; molecular diagnosis; international federation of gynecology and obstetrics; proof of concept; peritoneal washings; high throughput sequencing; next-generation sequencing; very elderly; human; female; article; peritoneal washing; early cancer diagnosis; tumor mutational burden; tumor-related gene; panel sequencing
Journal Title:	Gynecologic Oncology
Volume:	197
ISSN:	0090-8258
Publisher:	Elsevier Inc.
Date Published:	2025-06-01
Start Page:	155
End Page:	162
Language:	English
DOI:	10.1016/j.ygyno.2025.05.002
PUBMED:	40347837
PROVIDER:	scopus
PMCID:	PMC12160577
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-105004438411&doi=10.1016%2fj.ygyno.2025.05.002&partnerID=40&md5=bc639c5b6d27aec2b9aec019eb19dcce
Notes:	The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PDF. Corresponding MSK author is Britta Weigelt -- Source: Scopus