Protocol for assessing antibody-dependent cellular phagocytosis by primary murine and human macrophages Journal Article

  


Authors: Sevilla, C. M.; Mijacika, A.; Somoza, B.; Osorio, J. C.
Article Title: Protocol for assessing antibody-dependent cellular phagocytosis by primary murine and human macrophages
Abstract: Macrophages play a crucial role in mediating antibody-dependent cellular phagocytosis (ADCP), a process that enhances the effectiveness of several cancer immunotherapies. In this protocol, we outline detailed steps for isolating, differentiating, and polarizing macrophages from both mouse and human sources. Additionally, we describe an optimized technique to assess ADCP using in vitro co-culture studies followed by flow cytometry analysis. This protocol offers a reliable approach to evaluate the efficiency of macrophage-mediated phagocytosis of cancer cells during treatment with antibody-based therapies. For complete details on the use and execution of this protocol, please refer to Osorio et al.1 © 2025 The Authors
Keywords: flow cytometry; immunology; antibody; cancer
Journal Title: STAR Protocols
Volume: 6
Issue: 2
ISSN: 2666-1667
Publisher: Cell Press  
Date Published: 2025-06-20
Start Page: 103787
Language: English
DOI: 10.1016/j.xpro.2025.103787
PROVIDER: scopus
PUBMED: 40279242
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-105003290380&doi=10.1016%2fj.xpro.2025.103787&partnerID=40&md5=93a5510047dfa7784119eb693dfd9396
Notes: The MSK Cancer Center Support Grant (P30 CA008748) is acknowledge in the PDF -- Corresponding authors is MSK author: Juan C. Osorio -- Source: Scopus
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MSK Authors
  1. Juan Carlos Osorio
    15 Osorio
  2. Carlo miguel Ayala Sevilla
    2 Sevilla
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