Fission yeast metabolome dynamics during phosphate starvation and replenishment Journal Article
| Authors: | Sanchez, A. M.; Kyaw, A. K.; Violante, S. N.; Garg, A.; Cross, J. R.; Shuman, S. |
| Article Title: | Fission yeast metabolome dynamics during phosphate starvation and replenishment |
| Abstract: | Inorganic phosphate is an essential nutrient acquired by cells from their environment and assimilated into myriad intracellular metabolites and macromolecules. Here, we characterize the metabolic responses of fission yeast to a 24 h interval of phosphate starvation, during which cells enter a state of G0 quiescence. Time-resolved profiling revealed that many key phosphometabolites were progressively depleted, including (i) NTPs, NDPs, and dNTPs; (ii) coenzyme A, NAD+, NADP+, NADH, and ADP-ribose; (iii) glycolysis pathway intermediates upstream of pyruvate; (iv) pentose phosphate pathway intermediates from 6-phosphogluconate to sedoheptulose-7-phosphate; (v) nucleotide sugars GDP-hexose, UDP-glucose/galactose, and UDP-GalNAc/GlcNAc; and (vi) phospholipid precursors glycerol-3-phosphate, CDP-choline, and glycerophosphocholine. By contrast, early Krebs cycle intermediates accumulated during phosphate starvation. Other metabolic changes included the following: (i) interdiction of de novo pyrimidine synthesis; (ii) depletion of S-adenosylmethionine and S-adenosylhomocysteine; (iii) transient accumulation of polyamine biosynthetic intermediates putrescine and 5-methylthioadenosine; (iv) accumulation of betaine (correlating with an increase in expression of atd1 mRNA encoding aldehyde dehydrogenase); and (v) depletion of aminoadipate pathway intermediates 2-oxoadipate, 2-aminoadipate, and saccharopine. Replenishing phosphate after 24 h of starvation resulted in restoration of the pre-starvation metabolome (over 2 to 12 h) as cells exited quiescence and resumed growth. Copyright © 2025 Sanchez et al. |
| Keywords: | genetics; metabolism; phosphate; phosphates; schizosaccharomyces; schizosaccharomyces pombe; metabolomics; phosphate metabolism; phosphate starvation; metabolome |
| Journal Title: | mBio |
| Volume: | 16 |
| Issue: | 4 |
| ISSN: | 2150-7511 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2025-04-01 |
| Language: | English |
| DOI: | 10.1128/mbio.00241-25 |
| PUBMED: | 39998228 |
| PROVIDER: | scopus |
| PMCID: | PMC11980565 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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