Cryo-EM structures of the Spo11 core complex bound to DNA Journal Article

   


Authors: Yu, Y.; Wang, J.; Liu, K.; Zheng, Z.; Arter, M.; Claeys Bouuaert, C.; Pu, S.; Patel, D. J.; Keeney, S.
Article Title: Cryo-EM structures of the Spo11 core complex bound to DNA
Abstract: DNA double-strand breaks that initiate meiotic recombination are formed by the topoisomerase-relative enzyme Spo11, supported by conserved auxiliary factors. Because high-resolution structural data have not been available, many questions remain about the architecture of Spo11 and its partners and how they engage with DNA. We report cryo-electron microscopy structures at up to 3.3-Å resolution of DNA-bound core complexes of Saccharomyces cerevisiae Spo11 with Rec102, Rec104 and Ski8. In these structures, monomeric core complexes make extensive contacts with the DNA backbone and with the recessed 3′-OH and first 5′ overhanging nucleotide, establishing the molecular determinants of DNA end-binding specificity and providing insight into DNA cleavage preferences in vivo. The structures of individual subunits and their interfaces, supported by functional data in yeast, provide insight into the role of metal ions in DNA binding and uncover unexpected structural variation in homologs of the Top6BL component of the core complex. © The Author(s) 2024.
Keywords: controlled study; nonhuman; protein conformation; metabolism; protein protein interaction; protein binding; chemistry; dna; saccharomyces cerevisiae; double stranded dna break; models, molecular; saccharomyces cerevisiae proteins; saccharomyces cerevisiae protein; protein structure; dna binding; ultrastructure; molecular model; dna cleavage; dna topoisomerase; nucleotide; deoxyribonuclease; endodeoxyribonucleases; dna, fungal; cryoelectron microscopy; meiotic recombination; fungal dna; article; spo11 protein, s cerevisiae
Journal Title: Nature Structural and Molecular Biology
Volume: 32
ISSN: 1545-9993
Publisher: Nature Publishing Group  
Date Published: 2025-01-01
Start Page: 113
End Page: 124
Language: English
DOI: 10.1038/s41594-024-01382-8
PUBMED: 39304764
PROVIDER: scopus
PMCID: PMC11746154
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85204491552&doi=10.1038%2fs41594-024-01382-8&partnerID=40&md5=8fc658ad97436cd44686b4dee3d5c6db
Notes: The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PubMed record and PDF. Corresponding MSK author is Scott Keeney -- Source: Scopus
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MSK Authors
  1. Scott N Keeney
    138 Keeney
  2. Dinshaw J Patel
    479 Patel
  3. Corentin Francois Claeys Bouuaert
    7 Claeys Bouuaert
  4. Juncheng Wang
    12 Wang
  5. Stephen Pu
    5 Pu
  6. You Yu
    8 Yu
  7. Meret Regula Arter
    5 Arter
  8. Kaixian Liu
    6 Liu
  9. Zhi Zheng
    3 Zheng
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