Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2 Journal Article
| Authors: | Junqueira Alves, C.; Hannah, T.; Sadia, S.; Kolsteeg, C.; Dixon, A.; Wiener, R. J.; Nguyen, H.; Tipping, M. J.; Silva Ladeira, J.; Fernandes da Costa Franklin, P.; de Paula Dutra de Nigro, N.; Alves Dias, R.; Zabala Capriles, P. V.; Rodrigues Furtado de Mendonça, J. P.; Slesinger, P. A.; Costa, K. D.; Zou, H.; Friedel, R. H. |
| Article Title: | Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2 |
| Abstract: | Glioblastoma (GBM) is a malignant brain tumor with diffuse infiltration. Here, we demonstrate how GBM cells usurp guidance receptor Plexin-B2 for confined migration through restricted space. Using live-cell imaging to track GBM cells negotiating microchannels, we reveal endocytic vesicle accumulation at cell front and filamentous actin assembly at cell rear in a polarized manner. These processes are interconnected and require Plexin-B2 signaling. We further show that Plexin-B2 governs membrane tension and other membrane features such as endocytosis, phospholipid composition, and inner leaflet surface charge, thus providing biophysical mechanisms by which Plexin-B2 promotes GBM invasion. Together, our studies unveil how GBM cells regulate membrane tension and mechano-electrical coupling to adapt to physical constraints and achieve polarized confined migration. © The Author(s) 2024. |
| Keywords: | signal transduction; controlled study; unclassified drug; human cell; genetics; brain tumor; glioma; brain neoplasms; metabolism; actin; gene expression; embryo; protein assembly; molecular dynamics; nerve tissue proteins; protein; cell motion; pathology; cell line, tumor; physiology; regulatory mechanism; brain; glioma cell; glioblastoma; tumor cell line; cell membrane; cell migration; cell movement; cell polarity; neoplasm invasiveness; tumor; actins; involution; nerve protein; tension; endocytosis; cell invasion; extracellular space; molecular model; electric activity; phospholipid; biophysics; lipid composition; cell vacuole; mechanotransduction; cell; surface charge; actin filament; tumor invasion; humans; human; article; plexin; live cell imaging; inner membrane; internalization (cell); plexin b2; plxnb2 protein, human |
| Journal Title: | Nature Communications |
| Volume: | 16 |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2025-01-02 |
| Start Page: | 272 |
| Language: | English |
| DOI: | 10.1038/s41467-024-55056-6 |
| PUBMED: | 39747004 |
| PROVIDER: | scopus |
| PMCID: | PMC11697315 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
