NBAtlas: A harmonized single-cell transcriptomic reference atlas of human neuroblastoma tumors Journal Article

  

Authors:	Bonine, N.; Zanzani, V.; Van Hemelryk, A.; Vanneste, B.; Zwicker, C.; Thoné, T.; Roelandt, S.; Bekaert, S. L.; Koster, J.; Janoueix-Lerosey, I.; Thirant, C.; Van Haver, S.; Roberts, S. S.; Mus, L. M.; De Wilde, B.; Van Roy, N.; Everaert, C.; Speleman, F.; Vermeirssen, V.; Scott, C. L.; De Preter, K.
Article Title:	NBAtlas: A harmonized single-cell transcriptomic reference atlas of human neuroblastoma tumors
Abstract:	Neuroblastoma, a rare embryonic tumor arising from neural crest development, is responsible for 15% of pediatric cancer-related deaths. Recently, several single-cell transcriptome studies were performed on neuroblastoma patient samples to investigate the cell of origin and tumor heterogeneity. However, these individual studies involved a small number of tumors and cells, limiting the conclusions that could be drawn. To overcome this limitation, we integrated seven single-cell or single-nucleus datasets into a harmonized cell atlas covering 362,991 cells across 61 patients. We use this atlas to decipher the transcriptional landscape of neuroblastoma at single-cell resolution, revealing associations between transcriptomic profiles and clinical outcomes within the tumor compartment. In addition, we characterize the complex immune-cell landscape and uncover considerable heterogeneity among tumor-associated macrophages. Finally, we showcase the utility of our atlas as a resource by expanding it with additional data and using it as a reference for data-driven cell- type annotation.
Keywords:	dendritic cells; macrophages; model; reveals; gene-expression; markers; landscape; phenotypes; seq; disease-associated microglia
Journal Title:	Cell Reports
Volume:	43
Issue:	10
ISSN:	2211-1247
Publisher:	Cell Press
Date Published:	2024-10-22
Start Page:	114804
Language:	English
ACCESSION:	WOS:001334297500001
DOI:	10.1016/j.celrep.2024.114804
PROVIDER:	wos
PUBMED:	39368085
Notes:	Article -- 114804 -- Source: Wos

https://synapse.mskcc.org/synapse/works/has_related_data_chk/220119