Curcumin attenuates smoking and drinking activated NF-κB/IL-6 inflammatory signaling axis in cervical cancer Journal Article
| Authors: | Kashyap, V. K.; Nagesh, P. K. B.; Singh, A. K.; Massey, A.; Darkwah, G. P.; George, A.; Khan, S.; Hafeez, B. B.; Zafar, N.; Kumar, S.; Sinha, N.; Yallapu, M. M.; Jaggi, M.; Chauhan, S. C. |
| Article Title: | Curcumin attenuates smoking and drinking activated NF-κB/IL-6 inflammatory signaling axis in cervical cancer |
| Abstract: | Background: High-risk strains of HPV are known to cause cervical cancer. Multiple clinical studies have emphasized that smoking and drinking are critical risk factors for cervical cancer and its high-grade precursors. In this study, we investigated if smoking and/or drinking augment the molecular mechanisms of cervical carcinogenesis and defined a potential therapeutic approach for their attenuation. Methods: The impact of benzo[a]pyrene (B[a]P) and/or ethanol (EtOH) exposure on cervical cancer cells was assessed by measuring changes in their cell migration and invasion characteristics. Expression of HPV16 E6/E7, NF-κB, cytokines, and inflammation mediators was determined using qRT-PCR, immunoblotting, ELISA, luciferase reporter assay, and confocal microscopy. Herein, we used curcumin (Cur), and PLGA nanoparticle formulation of curcumin (PLGA-Cur) and determined effectiveness of free Cur and PLGA-Cur formulation on smoking and drinking activated NF-κB/IL-6 mediated inflammatory signaling pathways using in vitro cervical cancer models. Results: Treatments with B[a]P and/or EtOH altered the expression of HPV16 E6/E7 oncogenes and EMT markers in cervical cancer cells; it also enhanced migration and invasion. In addition, B[a]P and/or EtOH exposure promoted inflammation pathways through TNF-α and NF-κB signaling, leading to IL-6 upregulation and activation of VEGF. The molecular effects caused by B[a]P and/or EtOH exposure were effectively attenuated by curcumin (Cur)/PLGA-Cur treatment. Conclusions: These data suggest a molecular link between smoking, drinking, and HPV infectivity in cervical carcinogenesis. In addition, attenuation of these effects by treatment with Cur/PLGA-Cur treatment, implies the role of curcumin in cervical cancer prevention and treatment. © The Author(s) 2024. |
| Keywords: | benzo[a]pyrene; cervical cancer; nf-κb; cigarette smoking and drinking; cur/plga-cur; hpv16 e6/e7; nanoformulation |
| Journal Title: | Cancer Cell International |
| Volume: | 24 |
| ISSN: | 1475-2867 |
| Publisher: | Biomed Central Ltd |
| Date Published: | 2024-10-20 |
| Start Page: | 343 |
| Language: | English |
| DOI: | 10.1186/s12935-024-03513-z |
| PROVIDER: | scopus |
| PMCID: | PMC11492755 |
| PUBMED: | 39428480 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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