Noninvasive diagnostic method to objectively measure olfaction and diagnose smell disorders by a molecularly targeted fluorescence imaging agent Journal Article
| Authors: | Adilbay, D.; Gonzales, J.; Zazhytska, M.; Demetrio de Souza Franca, P.; Roberts, S.; Viray, T. D.; Artschwager, R.; Patel, S.; Kodra, A.; Overdevest, J. B.; Chow, C. Y.; King, G. F.; Jain, S. K.; Ordonez, A. A.; Carroll, L. S.; Lomvardas, S.; Reiner, T.; Pillarsetty, N. |
| Article Title: | Noninvasive diagnostic method to objectively measure olfaction and diagnose smell disorders by a molecularly targeted fluorescence imaging agent |
| Abstract: | Despite the recent advances in understanding the mechanisms of olfaction, no tools are currently available to noninvasively identify loss of smell. Because of the substantial increase in patients presenting with coronavirus disease 2019–related loss of smell, the pandemic has highlighted the urgent need to develop quantitative methods. Methods: Our group investigated the use of a novel fluorescent probe named Tsp1a-IR800P as a tool to diagnose loss of smell. Tsp1aIR800P targets sodium channel 1.7, which plays a critical role in olfaction by aiding the signal propagation to the olfactory bulb. Results: Intuitively, we have identified that conditions leading to loss of smell, including chronic inflammation and coronavirus disease 2019, correlate with the downregulation of sodium channel 1.7 expression in the olfactory epithelium, both at the transcript and at the protein levels. We demonstrated that lower Tsp1a-IR800P fluorescence emissions significantly correlate with loss of smell in live animals—thus representing a potential tool for its semiquantitative assessment. Currently available methods rely on delayed subjective behavioral studies. Conclusion: This method could aid in significantly improving preclinical and clinical studies by providing a way to objectively diagnose loss of smell and therefore aid the development of therapeutic interventions. © 2024 by the Society of Nuclear Medicine and Molecular Imaging. |
| Keywords: | immunohistochemistry; adult; clinical article; controlled study; human tissue; aged; histopathology; nonhuman; caloric intake; mass spectrometry; mouse; animal; animals; mice; gene expression; image analysis; odor; fluorescent dye; fluorescent dyes; animal experiment; animal model; immunofluorescence; molecular imaging; diagnostic imaging; chemistry; fluorescence microscopy; down regulation; high performance liquid chromatography; non invasive procedure; fluorescence imaging; olfactory bulb; ketamine; anosmia; thiamazole; optical imaging; olfactory receptor; procedures; olfactory nerve; optical; pentobarbital; smelling disorder; olfaction disorders; humans; human; male; female; article; smelling; smell; olfaction; olfactory epithelium; xylazine; coronavirus disease 2019; covid-19 |
| Journal Title: | Journal of Nuclear Medicine |
| Volume: | 65 |
| Issue: | 8 |
| ISSN: | 0161-5505 |
| Publisher: | Society of Nuclear Medicine |
| Date Published: | 2024-08-01 |
| Start Page: | 1293 |
| End Page: | 1300 |
| Language: | English |
| DOI: | 10.2967/jnumed.123.266123 |
| PUBMED: | 38960711 |
| PROVIDER: | scopus |
| PMCID: | PMC11294062 |
| DOI/URL: | |
| Notes: | The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PDF. Corresponding MSK author is Nagavarakishore Pillarsetty -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work