Site-specific bioconjugation and nuclear imaging Review
| Authors: | Sebastiano, J.; Samuels, Z. V.; Kao, W. S.; Zeglis, B. M. |
| Review Title: | Site-specific bioconjugation and nuclear imaging |
| Abstract: | Monoclonal antibodies and antibody fragments have proven to be highly effective vectors for the delivery of radionuclides to target tissues for positron emission tomography (PET) and single-photon emission computed tomography (SPECT). However, the stochastic methods that have traditionally been used to attach radioisotopes to these biomolecules inevitably produce poorly defined and heterogeneous probes and can impair the ability of the immunoglobulins to bind their molecular targets. In response to this challenge, an array of innovative site-specific and site-selective bioconjugation strategies have been developed, and these approaches have repeatedly been shown to yield better-defined and more homogeneous radioimmunoconjugates with superior in vivo performance than their randomly modified progenitors. In this Current Opinion in Chemical Biology review, we will examine recent advances in this field, including the development — and, in some cases, clinical translation — of nuclear imaging agents radiolabeled using strategies that target the heavy chain glycans, peptide tags, and unnatural amino acids. © 2024 The Authors |
| Keywords: | protein expression; unclassified drug; review; nonhuman; bone metastasis; positron emission tomography; computer assisted tomography; peptide; molecular imaging; diagnostic imaging; monoclonal antibody; xenograft; radiopharmaceutical agent; amino acid; antibody; chemical reaction; zirconium 89; glycan derivative; click chemistry; programmed death 1 ligand 1; non small cell lung cancer; conjugation; antibody conjugate; single photon emission computed tomography; thiol derivative; antibody fragment; human epidermal growth factor receptor 2 positive breast cancer; heavy chain; radiolabeling; human; unnatural amino acids; site-specific bioconjugation; positron emission tomography-computed tomography; heavy chain glycans; site-selective bioconjugation; sshn3 zr 89; thp apn09 ga 68 |
| Journal Title: | Current Opinion in Chemical Biology |
| Volume: | 81 |
| ISSN: | 1367-5931 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2024-08-01 |
| Start Page: | 102471 |
| Language: | English |
| DOI: | 10.1016/j.cbpa.2024.102471 |
| PUBMED: | 38833913 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Review -- Source: Scopus |
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