TCTP regulates genotoxic stress and tumorigenicity via intercellular vesicular signaling Journal Article
| Authors: | Amson, R.; Senff-Ribeiro, A.; Karafin, T.; Lespagnol, A.; Honoré, J.; Baylot, V.; Banroques, J.; Tanner, N. K.; Chamond, N.; Dimitrov, J. D.; Hoebeke, J.; Droin, N. M.; Job, B.; Piard, J.; Bommer, U. A.; Choi, K. W.; Abdelfatah, S.; Efferth, T.; Telerman, S. B.; Geyer, F. C.; Reis-Filho, J.; Telerman, A. |
| Article Title: | TCTP regulates genotoxic stress and tumorigenicity via intercellular vesicular signaling |
| Abstract: | Oncogenic intercellular signaling is regulated by extracellular vesicles (EVs), but the underlying mechanisms remain mostly unclear. Since TCTP (translationally controlled tumor protein) is an EV component, we investigated whether it has a role in genotoxic stress signaling and malignant transformation. By generating a Tctp-inducible knockout mouse model (Tctp–/f–), we report that Tctp is required for genotoxic stress-induced apoptosis signaling via small EVs (sEVs). Human breast cancer cells knocked-down for TCTP show impaired spontaneous EV secretion, thereby reducing sEV-dependent malignant growth. Since Trp53–/– mice are prone to tumor formation, we derived tumor cells from Trp53–/–;Tctp–/f– double mutant mice and describe a drastic decrease in tumori-genicity with concomitant decrease in sEV secretion and content. Remarkably, Trp53–/–;Tctp–/f– mice show highly prolonged survival. Treatment of Trp53–/– mice with sertraline, which inhibits TCTP function, increases their survival. Mechanistically, TCTP binds DDX3, recruiting RNAs, including miRNAs, to sEVs. Our findings establish TCTP as an essential protagonist in the regulation of sEV-signaling in the context of apoptosis and tumorigenicity. © The Author(s) 2024.; (Figure presented.) Signaling via small extracellular vesicles (sEVs) is crucial for the regulation of cell fate. This study shows that TCTP is an essential component of sEVs, regulating their secretion, protein and RNA content, affecting thereby both apoptosis and cancer. Tctp–/f– sEVs have impaired cell death signaling capacity upon genotoxic stress. Deletion of Tctp reprograms/reverts Trp53–/–-driven tumorigenesis. Tumor-derived sEVs promote malignant transformation in a TCTP-dependent manner. TCTP binds DDX3 to regulate the transport of microRNAs into sEVs. © The Author(s) 2024. |
| Keywords: | signal transduction; neoplasm; neoplasms; mouse; animal; metabolism; animals; mice; apoptosis; signaling; pathology; tumor marker; bystander effect; humans; human; biomarkers, tumor; small extracellular vesicles (sevs); tumor reprogramming; tumor reversion |
| Journal Title: | EMBO Reports |
| Volume: | 25 |
| Issue: | 4 |
| ISSN: | 1469-221X |
| Publisher: | Wiley Blackwell |
| Date Published: | 2024-04-12 |
| Start Page: | 1962 |
| End Page: | 1986 |
| Language: | English |
| DOI: | 10.1038/s44319-024-00108-7 |
| PUBMED: | 38548973 |
| PROVIDER: | scopus |
| PMCID: | PMC11014985 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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