First-in-human evaluation of site-specifically labeled (89)Zr-pertuzumab in patients with HER2-positive breast cancer Journal Article
| Authors: | Yeh, R.; O'Donoghue, J. A.; Jayaprakasam, V. S.; Mauguen, A.; Min, R.; Park, S.; Brockway, J. P.; Bromberg, J. F.; Zhi, W. I.; Robson, M. E.; Sanford, R.; Modi, S.; Agnew, B. J.; Lyashchenko, S. K.; Lewis, J. S.; Ulaner, G. A.; Zeglis, B. M. |
| Article Title: | First-in-human evaluation of site-specifically labeled (89)Zr-pertuzumab in patients with HER2-positive breast cancer |
| Abstract: | Radioimmunoconjugates targeting human epidermal growth factor receptor 2 (HER2) have shown potential to noninvasively visualize HER2-positive tumors. However, the stochastic approach that has been traditionally used to radiolabel these antibodies yields poorly defined and heterogeneous products with suboptimal in vivo performance. Here, we describe a first-in-human PET study on patients with HER2-positive breast cancer evaluating the safety, biodistribution, and dosimetry of 89Zr-site-specific (ss)-pertuzumab PET, a site-specifically labeled radioimmunoconjugate designed to circumvent the limitations of random stochastic lysine labeling. Methods: Six patients with HER2-positive metastatic breast cancer were enrolled in a prospective clinical trial. Pertuzumab was site-specifically modified with desferrioxamine (DFO) via a novel chemoenzymatic strategy and subsequently labeled with 89Zr. Patients were administered 74 MBq of 89Zr-ss-pertuzumab in 20 mg of total antibody intravenously and underwent PET/CT at 1 d, 3–4 d, and 5–8 d after injection. PET imaging, whole-body probe counts, and blood draws were performed to assess the pharmacokinetics, biodistribution, and dosimetry. Results: 89Zr-ss-pertuzumab PET/CT was used to assess HER2 status and heterogeneity to guide biopsy and decide the next line of treatment at progression. The radioimmunoconjugate was able to detect known sites of malignancy, suggesting that these tumor lesions were HER2-positive. The optimal imaging time point was 5–8 d after administration, and no toxicities were observed. Dosimetry estimates from OLINDA showed that the organs receiving the highest doses (mean 6 SD) were kidney (1.8 6 0.5 mGy/MBq), liver (1.7 6 0.3 mGy/MBq), and heart wall (1.2 6 0.1 mGy/MBq). The average effective dose for 89Zr-ss-pertuzu-mab was 0.54 6 0.03 mSv/MBq, which was comparable to both stochastically lysine-labeled 89Zr-DFO-pertuzumab and 89Zr-DFO-tras-tuzumab. One patient underwent PET/CT with both 89Zr-ss-pertuzumab and 89Zr-DFO-pertuzumab 1 mo apart, with 89Zr-ss-pertuzumab demonstrating improved lesion detection and higher tracer avidity. Conclusion: This study demonstrated the safety, dosimetry, and potential clinical applications of 89Zr-ss-pertuzumab PET/CT. 89Zr-ss-pertuzumab may detect more lesions than 89Zr-DFO-pertuzumab. Potential clinical applications include real-time evaluation of HER2 status to guide biopsy and assist in treatment decisions. COPYRIGHT © 2024 by the Society of Nuclear Medicine and Molecular Imaging. |
| Keywords: | adult; clinical article; aged; unclassified drug; drug safety; positron emission tomography; prospective study; prospective studies; breast cancer; diagnostic imaging; breast neoplasms; monoclonal antibody; drug distribution; isotope labeling; tissue distribution; dosimetry; breast tumor; tracer; metastatic breast cancer; lysine; her2; pertuzumab; antibody conjugate; immunoconjugates; immuno-pet; antibodies, monoclonal, humanized; radioimmunoconjugates; human epidermal growth factor receptor 2 positive breast cancer; humans; human; male; female; article; positron emission tomography-computed tomography; positron emission tomography computed tomography; zirconium pertuzumab zr 89 |
| Journal Title: | Journal of Nuclear Medicine |
| Volume: | 65 |
| Issue: | 3 |
| ISSN: | 0161-5505 |
| Publisher: | Society of Nuclear Medicine |
| Date Published: | 2024-03-01 |
| Start Page: | 386 |
| End Page: | 393 |
| Language: | English |
| DOI: | 10.2967/jnumed.123.266392 |
| PUBMED: | 38272704 |
| PROVIDER: | scopus |
| PMCID: | PMC10924157 |
| DOI/URL: | |
| Notes: | Article -- Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PDF -- MSK corresponding author is Randy Yeh -- Source: Scopus Source: Scopus |
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