Factors governing the transcriptome changes and chronological lifespan of fission yeast during phosphate starvation Journal Article
| Authors: | Garg, A.; Sanchez, A. M.; Schwer, B.; Shuman, S. |
| Article Title: | Factors governing the transcriptome changes and chronological lifespan of fission yeast during phosphate starvation |
| Abstract: | Starvation of Schizosaccharomyces pombe for inorganic phosphate elicits adaptive transcriptome changes in which mRNAs driving ribosome biogenesis, tRNA biogenesis, and translation are globally downregulated, while those for autophagy and phosphate mobilization are upregulated. Here, we interrogated three components of the starvation response: upregulated autophagy; the role of transcription factor Pho7 (an activator of the PHO regulon); and upregulated expression of ecl3, one of three paralogous genes (ecl1, ecl2, and ecl3) collectively implicated in cell survival during other nutrient stresses. Ablation of autophagy factor Atg1 resulted in early demise of phosphate-starved fission yeast, as did ablation of Pho7. Transcriptome profiling of phosphate-starved pho7Δ cells highlighted Pho7 as an activator of genes involved in phosphate acquisition and mobilization, not limited to the original three-gene PHO regulon, and additional starvation-induced genes (including ecl3) not connected to phosphate dynamics. Pho7-dependent gene induction during phosphate starvation tracked with the presence of Pho7 DNA-binding elements in the gene promoter regions. Fewer ribosome protein genes were downregulated in phosphate-starved pho7Δ cells versus WT, which might contribute to their shortened lifespan. An ecl3Δ mutant elicited no gene expression changes in phosphate-replete cells and had no impact on survival during phosphate starvation. By contrast, pan-ecl deletion (ecl123Δ) curtailed lifespan during chronic phosphate starvation. Phosphate-starved ecl123Δ cells experienced a more widespread downregulation of mRNAs encoding aminoacyl tRNA synthetases vis-à-vis WT or pho7Δ cells. Collectively, these results enhance our understanding of fission yeast phosphate homeostasis and survival during nutrient deprivation. © 2024 The Authors |
| Keywords: | signal transduction; controlled study; unclassified drug; gene sequence; single nucleotide polymorphism; nonhuman; flow cytometry; cell death; dna damage; cell survival; gene expression; transcription factor; proteomics; transcriptomics; transcription factors; rna binding protein; biosynthesis; protein synthesis; microarray analysis; chromatin immunoprecipitation; transcription; immunoblotting; binding site; dna sequence; down regulation; upregulation; yeast; dna binding; homeostasis; cell cycle regulation; phosphate; transcriptome; ablation therapy; rna extraction; ribosomal proteins; biogenesis; lifespan; bacterium isolation; rna sequence; northern blotting; schizosaccharomyces pombe; starvation; expansion; fission yeast; southern blotting; ablation; ribosome; chronology; autophagosome; translation factors; ribosomal protein; macromolecules; osmotic stress; nutrients; synthetases; article; rna sequencing; rna 28s; phosphate starvation; phosphate starvation response; autophagy (cellular); phosphate deficiency; aminoacyl trna synthetase; transcription factor pho7; trna biogenesis; aminoacyl; starvation response |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 300 |
| Issue: | 3 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2024-03-01 |
| Start Page: | 105718 |
| Language: | English |
| DOI: | 10.1016/j.jbc.2024.105718 |
| PUBMED: | 38311173 |
| PROVIDER: | scopus |
| PMCID: | PMC10910108 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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