The evolutionary loss of the Eh1 motif in FoxE1 in the lineage of placental mammals Journal Article
| Authors: | Sharma, M.; Larow, V. M.; Dobychina, N.; Kessler, D. S.; Krasilnikova, M. M.; Yaklichkin, S. |
| Article Title: | The evolutionary loss of the Eh1 motif in FoxE1 in the lineage of placental mammals |
| Abstract: | Forkhead box E1 (FoxE1) protein is a transcriptional regulator known to play a major role in the development of the thyroid gland. By performing sequence alignments, we detected a deletion in FoxE1, which occurred in the evolution of mammals, near the point of divergence of placental mammals. This deletion led to the loss of the majority of the Eh1 motif, which was important for interactions with transcriptional corepressors. To investigate a potential mechanism for this deletion, we analyzed replication through the deletion area in mammalian cells with two-dimensional gel electrophoresis, and in vitro, using a primer extension reaction. We demonstrated that the area of the deletion presented an obstacle for replication in both assays. The exact position of polymerization arrest in primer extension indicated that it was most likely caused by a quadruplex DNA structure. The quadruplex structure hypothesis is also consistent with the exact borders of the deletion. The exact roles of these evolutionary changes in FoxE1 family proteins are still to be determined. © 2023 Sharma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Keywords: | controlled study; gene sequence; sequence analysis; genetics; nonhuman; forkhead transcription factors; animal; metabolism; animals; gene expression; amino acid sequence; genetic transfection; sequence alignment; nucleotide sequence; escherichia coli; polymerization; gene duplication; pregnancy; dna sequence; dna structure; dna replication origin; thyroid gland; forkhead transcription factor; placenta; mammal cell; open reading frame; agar gel electrophoresis; evolutionary adaptation; phylogenetic tree; eutheria; physiological stress; sanger sequencing; female; article; placental mammal |
| Journal Title: | PLoS ONE |
| Volume: | 18 |
| Issue: | 12 |
| ISSN: | 1932-6203 |
| Publisher: | Public Library of Science |
| Date Published: | 2023-12-27 |
| Start Page: | e0296176 |
| Language: | English |
| DOI: | 10.1371/journal.pone.0296176 |
| PUBMED: | 38150428 |
| PROVIDER: | scopus |
| PMCID: | PMC10752562 |
| DOI/URL: | |
| Notes: | Source: Scopus |
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