Ex vivo pleural effusion cultures to study chimeric antigen receptor T cell cytotoxicity in an immunocompetent environment Journal Article

   


Authors: Tano, Z. E.; Kiesgen, S.; Chintala, N. K.; Dozier, J.; Quach, H. T.; Messinger, J.; Tan, K. S.; Adusumilli, P. S.
Article Title: Ex vivo pleural effusion cultures to study chimeric antigen receptor T cell cytotoxicity in an immunocompetent environment
Abstract: Current in vitro and in vivo assays used to study immunotherapeutic interventions lack human immune components that mimic the tumor microenvironment to investigate drug potency and limitations of efficacy. Herein, we describe an ex vivo pleural effusion culture (ePEC) assay, using malignant pleural-effusion-derived soluble and cellular factors that differentially affected the cytotoxicity of chimeric antigen receptor (CAR) T cells. Following identification of CAR T cell-suppressive factors, blocking of individual factors reveals their contribution to compromising T cell efficacy. ePEC is a human component assay that can be utilized for developing next-generation cell and antibody therapies that counteract immunosuppression. © 2023 The Authors
Keywords: adult; human tissue; aged; human cell; genetics; t lymphocyte; t-lymphocytes; cytotoxicity; in vitro study; immunotherapy; bioassay; chimeric antigen receptor; cell therapy; clinical effectiveness; pleural effusion, malignant; ex vivo study; adoptive immunotherapy; immunotherapy, adoptive; immunocompetence; tumor microenvironment; malignant pleura effusion; humans; human; male; female; article; receptors, chimeric antigen; cp: immunology; cp: cancer biology
Journal Title: Cell Reports Methods
Volume: 3
Issue: 11
ISSN: 2667-2375
Publisher: Cell Press  
Date Published: 2023-11-20
Start Page: 100622
Language: English
DOI: 10.1016/j.crmeth.2023.100622
PUBMED: 37875122
PROVIDER: scopus
PMCID: PMC10694486
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85178285994&doi=10.1016%2fj.crmeth.2023.100622&partnerID=40&md5=87687e2a63df29faa6b035db9cefbe1c
Notes: Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PDF -- MSK corresponding author is Prasad Adusumilli -- Source: Scopus
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MSK Authors
  1. Prasad S Adusumilli
    496 Adusumilli
  2. Kay See Tan
    241 Tan
  3. Navin Kumar Chintala
    27 Chintala
  4. Zachary Tano
    11 Tano
  5. Jordan Michael Dozier
    9 Dozier
  6. Stefan Thomas Kiesgen
    5 Kiesgen
  7. John Conrad Messinger
    7 Messinger
  8. Tu Hue Quach
    8 Quach
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