Circulating microbial cell–free DNA is increased during neutropenia after hematopoietic stem cell transplantation Journal Article
| Authors: | Blair, L. M.; Akhund-Zade, J.; Katsamakis, Z. A.; Smibert, O. C.; Wolfe, A. E.; Giardina, P.; Slingerland, J.; Bercovici, S.; Perales, M. A.; Taur, Y.; van den Brink, M. R. M.; Peled, J. U.; Markey, K. A. |
| Article Title: | Circulating microbial cell–free DNA is increased during neutropenia after hematopoietic stem cell transplantation |
| Abstract: | We used a next-generation sequencing platform to characterize microbial cell–free DNA (mcfDNA) in plasma samples from patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HCT). In this observational study, we sought to characterize plasma mcfDNA in order to explore its potential association with the immunologic complications of transplantation. We compared serially collected patient samples with plasma collected from healthy control subjects. We observed changes in total mcfDNA burden in the plasma after transplantation, which was most striking during the early posttransplant neutropenic phase. This elevation could be attributed to a number of specific bacterial taxa, including Veillonella, Bacteroides, and Prevotella (genus level). For an additional cohort of patients, we compared the data of mcfDNA from plasma with 16s-ribosomal RNA sequencing data from stool samples collected at matched time points. In a number of patients, we confirmed that mcfDNA derived from specific microbial taxa (eg, Enterococcus) could also be observed in the matched stool sample. Quantification of mcfDNA may generate novel insights into mechanisms by which the intestinal microbiome influences systemic cell populations and, thus, has been associated with outcomes for patients with cancer. © 2023 by The American Society of Hematology. |
| Keywords: | mcfdna is increased during neutropenia after allo-hct.; studying the taxa-of-origin of this circulating dna may provide novel insights into post-hct biology |
| Journal Title: | Blood Advances |
| Volume: | 7 |
| Issue: | 21 |
| ISSN: | 2473-9529 |
| Publisher: | American Society of Hematology |
| Date Published: | 2023-11-14 |
| Start Page: | 6744 |
| End Page: | 6750 |
| Language: | English |
| DOI: | 10.1182/bloodadvances.2023010208 |
| PUBMED: | 37399491 |
| PROVIDER: | scopus |
| PMCID: | PMC10651422 |
| DOI/URL: | |
| Notes: | The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PDF -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
-
616Van Den Brink -
913Perales -
147Taur -
154Peled -
53Slingerland -
38Markey -
19Giardina
Related MSK Work