Cancer modeling by transgene electroporation in adult zebrafish (TEAZ) Journal Article
| Authors: | Montal, E.; Suresh, S.; Ma, Y.; Tagore, M. M.; White, R. M.; Amatruda, J. F.; Houart, C.; Kawakami, K.; Poss, K. D. |
| Article Title: | Cancer modeling by transgene electroporation in adult zebrafish (TEAZ) |
| Abstract: | Transgenic expression of genes is a mainstay of cancer modeling in zebrafish. Traditional transgenic techniques rely upon injection into one-cell embryos, but ideally these transgenes would be expressed only in adult somatic tissues. We provide a method to model cancer in adult zebrafish in which transgenes can be expressed via electroporation. Using melanoma as an example, we demonstrate the feasibility of expressing oncogenes such as BRAFV600E as well as CRISPR/Cas9 inactivation of tumor suppressors such as PTEN. These approaches can be performed in any genetic background such as existing fluorophore reporter lines or the casper line. These methods can readily be extended to other cell types allowing for rapid adult modeling of cancer in zebrafish. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
| Keywords: | genetics; neoplasm; neoplasms; animal; animals; melanoma; transgenic animal; oncogenes; transgene; electroporation; cancer modeling; transgenes; zebra fish; zebrafish; tumor suppressors; animals, genetically modified; crispr/cas9; somatic transgenesis; electroporation therapies; electroporation treatment |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 2707 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2024-01-01 |
| Start Page: | 83 |
| End Page: | 97 |
| Language: | English |
| DOI: | 10.1007/978-1-0716-3401-1_5 |
| PUBMED: | 37668906 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Book Chapter [5] in "[Zebrafish: Methods and Protocols 3rd. ed]" (ISBN: [978-1-0716-3400-4]) -- Source: Scopus |
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