Cancer modeling by transgene electroporation in adult zebrafish (TEAZ) Journal Article

Authors: Montal, E.; Suresh, S.; Ma, Y.; Tagore, M. M.; White, R. M.; Amatruda, J. F.; Houart, C.; Kawakami, K.; Poss, K. D.
Article Title: Cancer modeling by transgene electroporation in adult zebrafish (TEAZ)
Abstract: Transgenic expression of genes is a mainstay of cancer modeling in zebrafish. Traditional transgenic techniques rely upon injection into one-cell embryos, but ideally these transgenes would be expressed only in adult somatic tissues. We provide a method to model cancer in adult zebrafish in which transgenes can be expressed via electroporation. Using melanoma as an example, we demonstrate the feasibility of expressing oncogenes such as BRAFV600E as well as CRISPR/Cas9 inactivation of tumor suppressors such as PTEN. These approaches can be performed in any genetic background such as existing fluorophore reporter lines or the casper line. These methods can readily be extended to other cell types allowing for rapid adult modeling of cancer in zebrafish. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Keywords: genetics; neoplasm; neoplasms; animal; animals; melanoma; transgenic animal; oncogenes; transgene; electroporation; cancer modeling; transgenes; zebra fish; zebrafish; tumor suppressors; animals, genetically modified; crispr/cas9; somatic transgenesis; electroporation therapies; electroporation treatment
Journal Title: Methods in Molecular Biology
Volume: 2707
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2024-01-01
Start Page: 83
End Page: 97
Language: English
DOI: 10.1007/978-1-0716-3401-1_5
PUBMED: 37668906
PROVIDER: scopus
Notes: Book Chapter [5] in "[Zebrafish: Methods and Protocols 3rd. ed]" (ISBN: [978-1-0716-3400-4]) -- Source: Scopus
Citation Impact
MSK Authors
  1. Richard Mark White
    60 White
  2. Emily Dina Montal
    14 Montal
  3. Mohita Malay Tagore
    5 Tagore
  4. Shruthy Suresh
    7 Suresh
  5. Yilun Ma
    8 Ma