Simultaneous quantitative imaging of two PET radiotracers via the detection of positron–electron annihilation and prompt gamma emissions Journal Article
| Authors: | Pratt, E. C.; Lopez-Montes, A.; Volpe, A.; Crowley, M. J.; Carter, L. M.; Mittal, V.; Pillarsetty, N.; Ponomarev, V.; Udías, J. M.; Grimm, J.; Herraiz, J. L. |
| Article Title: | Simultaneous quantitative imaging of two PET radiotracers via the detection of positron–electron annihilation and prompt gamma emissions |
| Abstract: | In conventional positron emission tomography (PET), only one radiotracer can be imaged at a time, because all PET isotopes produce the same two 511 keV annihilation photons. Here we describe an image reconstruction method for the simultaneous in vivo imaging of two PET tracers and thereby the independent quantification of two molecular signals. This method of multiplexed PET imaging leverages the 350–700 keV range to maximize the capture of 511 keV annihilation photons and prompt γ-ray emission in the same energy window, hence eliminating the need for energy discrimination during reconstruction or for signal separation beforehand. We used multiplexed PET to track, in mice with subcutaneous tumours, the biodistributions of intravenously injected [124I]I-trametinib and 2-deoxy-2-[18F]fluoro-d-glucose, [124I]I-trametinib and its nanoparticle carrier [89Zr]Zr-ferumoxytol, and the prostate-specific membrane antigen (PSMA) and infused PSMA-targeted chimaeric antigen receptor T cells after the systemic administration of [68Ga]Ga-PSMA-11 and [124I]I. Multiplexed PET provides more information depth, gives new uses to prompt γ-ray-emitting isotopes, reduces radiation burden by omitting the need for an additional computed-tomography scan and can be implemented on preclinical and clinical systems without any modifications in hardware or image acquisition software. © 2023, The Author(s), under exclusive licence to Springer Nature Limited. |
| Keywords: | positron emission tomography; isotopes; mouse; animal; animals; mice; tomography, x-ray computed; antigens; radioactive iodine; iodine radioisotopes; computerized tomography; positron-emission tomography; quantitative imaging; radioactive tracers; photons; mammals; in-vivo imaging; electrons; image reconstruction methods; image reconstruction; positrons; electron; gamma rays; procedures; iodine-124; prostate-specific membrane antigens; male; molecular signals; x-ray computed tomography; tomography imaging; annihilation photons; gamma emission; positron-electron annihilation; prompt gammas |
| Journal Title: | Nature Biomedical Engineering |
| Volume: | 7 |
| Issue: | 8 |
| ISSN: | 2157-846X |
| Publisher: | Nature Publishing Group |
| Date Published: | 2023-08-01 |
| Start Page: | 1028 |
| End Page: | 1039 |
| Language: | English |
| DOI: | 10.1038/s41551-023-01060-y |
| PUBMED: | 37400715 |
| PROVIDER: | scopus |
| PMCID: | PMC10810307 |
| DOI/URL: | |
| Notes: | Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PDF -- MSK corresponding author is Jan Grimm -- Source: Scopus |
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